Animal Physiology Laboratory, Department of Life Sciences, Sciences Faculty of Sfax, University of Sfax, Sfax, Tunisia.
Ecology, Biology and Physiology Laboratory of Aquatic Organisms, Department of Biological Sciences, Sciences Faculty of Tunis, University of Tunis El Manar, 2092, Tunis, Tunisia.
Environ Sci Pollut Res Int. 2022 Aug;29(36):54827-54841. doi: 10.1007/s11356-022-19497-0. Epub 2022 Mar 21.
The current study was conducted to assess the beneficial effect of selenium (Se) on maneb-induced cardiotoxicity and fatty acid alterations in adult mice. Swiss albino male mice were assigned into four experimental groups. The first group consisted of negative controls. The second group represented the positive controls where mice received daily, via the diet, sodium selenite at a dose of 0.2 mg/kg. For the third group, mice were subjected to intraperitoneal injections of maneb (30 mg/kg BW). The fourth group (MB+Se) received daily the same dose of maneb as group 3 along with sodium selenite at the same dose as group 2. Mice exposure to maneb caused cardiotoxicity as indicated by an increase in malondialdehyde, hydrogen peroxide, and protein carbonyl levels, and an alteration of the antioxidant defense system (catalase, glutathione peroxidase, superoxide dismutase, glutathione, and vitamin C). Plasma lactate dehydrogenase activity and total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels increased, while high-density lipoprotein cholesterol level decreased. Results showed also a decrease in the amount of n-3 PUFA, docosahexaenoic, docosapentaenoic, and eicosapentaenoic acids. However, an increase in the levels of MUFA, cis-vaccenic, and palmitoleic acids was observed. Co-administration of Se restored the parameters indicated above to near control values. The histopathological findings confirmed the biochemical results. Selenium could be a useful and efficient agent against maneb-induced cardiotoxicity.
本研究旨在评估硒（Se）对代森锰诱发的成年小鼠心脏毒性和脂肪酸改变的有益作用。将瑞士白化雄性小鼠分为四组进行实验。第一组为阴性对照组。第二组为阳性对照组，每天通过饮食摄入亚硒酸钠，剂量为 0.2mg/kg。第三组小鼠接受代森锰（30mg/kgBW）的腹腔注射。第四组（MB+Se）接受与第三组相同剂量的代森锰，并每天给予与第二组相同剂量的亚硒酸钠。
暴露于代森锰会导致心脏毒性，表现为丙二醛、过氧化氢和蛋白质羰基水平升高，抗氧化防御系统（过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶、谷胱甘肽和维生素 C）发生改变。血浆乳酸脱氢酶活性以及总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平升高，而高密度脂蛋白胆固醇水平降低。结果还显示 n-3PUFA、二十二碳六烯酸、二十碳五烯酸和二十碳五烯酸的含量减少，而单不饱和脂肪酸（MUFA）、顺式-麦角烯酸和棕榈油酸的水平增加。
硒的共同给药可使上述参数恢复到接近对照值。组织病理学发现证实了生化结果。硒可能是一种对抗代森锰诱发的心脏毒性的有用且有效的药物。
