Chemistry and Physics, College of Science, Health, Engineering and Education, Murdoch University, WA 6150, Australia.
Chemistry and Physics, College of Science, Health, Engineering and Education, Murdoch University, WA 6150, Australia.
Colloids Surf B Biointerfaces. 2020 Apr;188:110751. doi: 10.1016/j.colsurfb.2019.110751. Epub 2019 Dec 23.
This work investigated the synthesis of carbohydrate functionalized methylene blue doped amine grafted mesoporous silica nanoparticles (MB AMSN) and their application in bioimaging. A single-pot synthesis methodology was developed via a modified co-condensation sol-gel technique for simultaneous incorporation of the dye molecule in the nanoparticles, with amine grafting for subsequent functionalization. The obtained nanoparticles (∼ 450 nm) are mesoporous and have a high surface area (538 m/g), pore-volume (0.3 cm/g), showed excellent UV-vis absorbance, and dye encapsulation efficiency (> 75 %). These fluorescent nanoparticles were further functionalized with carbohydrate molecules before application as contrast agents in bacterial cells. In the present study, gram-positive (E. coli) and gram-negative (B. subtilis) bacteria were used as model organisms. Confocal laser microscopy results showed that the nanoparticles are highly fluorescent, and SEM of glucose conjugated MB doped nanoparticles indicated close interaction with E. coli with no toxicity observed towards either bacterial cells. The results demonstrate that by suitable surface functionalization, the methylene blue doped silica nanoparticles can be used as bioimaging agents.
本工作研究了碳水化合物功能化亚甲蓝掺杂胺接枝介孔硅纳米粒子（MB-AMS-N）的合成及其在生物成像中的应用。通过改良的共缩合溶胶-凝胶技术开发了一种一锅合成方法，通过该方法可以同时将染料分子掺入纳米粒子中，并进行胺接枝以进行后续功能化。所得纳米粒子（约 450nm）为介孔纳米粒子，具有高比表面积（538m/g）、孔体积（0.3cm/g），表现出优异的紫外-可见吸收和染料包封效率（＞75%）。这些荧光纳米粒子在进一步用碳水化合物分子功能化后，可用作细菌细胞中的对比剂。在本研究中，革兰氏阳性（E. coli）和革兰氏阴性（B. subtilis）细菌被用作模型生物。共焦激光显微镜结果表明，纳米粒子具有很强的荧光性，SEM 观察到葡萄糖偶联 MB 掺杂纳米粒子与 E. coli 密切相互作用，对两种细菌均无毒性。结果表明，通过适当的表面功能化，亚甲蓝掺杂硅纳米粒子可用作生物成像剂。
