Märtlbauer E, Gareis M, Terplan G
Institute for Hygiene and Technology of Food of Animal Origin, University of Munich, Federal Republic of Germany.
Appl Environ Microbiol. 1988 Jan;54(1):225-30. doi: 10.1128/aem.54.1.225-230.1988.
Antisera against roridin A were prepared by using a roridin A-hemisuccinate derivative coupled to human serum albumin as the immunogen. Antibodies could be detected in the sera of the immunized rabbits as early as 4 weeks after the initial exposure. After one booster injection at week 14, high antibody titers were measured over a period of 21 weeks. The specificity and sensitivity of the antibodies were tested by using roridin A-hemisuccinate coupled to horseradish peroxidase as an enzyme-linked toxin in a competitive assay with a double-antibody solid phase. The assay was most specific for the tested macrocyclic trichothecenes, and the relative cross-reactivities with roridin A, roridin J, verrucarin A, satratoxin H, and satratoxin G were 1, 0.41, 0.15, 0.15, and 0.07, respectively. When 16 nonmacrocyclic trichothecenes were tested, only diacetylverrucarol (0.0015) and verrucarol (0.0005) showed minor cross-reactivity. The sensitivity of the enzyme immunoassay for the detection of roridin A was in the range of 5 to 50 ng/ml (0.16 to 1.6 ng per assay).
以与人类血清白蛋白偶联的罗里毒素A-半琥珀酸衍生物作为免疫原制备了抗罗里毒素A抗血清。早在初次接触后4周就能在免疫兔的血清中检测到抗体。在第14周进行一次加强注射后，在21周的时间里测量到了高抗体滴度。通过使用与辣根过氧化物酶偶联的罗里毒素A-半琥珀酸作为酶联毒素，在双抗体固相竞争测定中测试了抗体的特异性和敏感性。该测定对所测试的大环单端孢霉烯最为特异，与罗里毒素A、罗里毒素J、疣孢菌素A、葡萄穗霉毒素H和葡萄穗霉毒素G的相对交叉反应率分别为1、0.41、0.15、0.15和0.07。当测试16种非大环单端孢霉烯时，只有二乙酰疣孢菌素醇（0.0015）和疣孢菌素醇（0.0005）显示出轻微的交叉反应。酶免疫测定法检测罗里毒素A的灵敏度在5至50 ng/ml范围内（每次测定0.16至1.6 ng）。
