DTMUV 截短 E 蛋白可对雏鸭提供保护。

The truncated E protein of DTMUV provide protection in young ducks.

机构信息

Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Scientiﬁc Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture, Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Baishigang Road, Guangzhou, Guangdong, PR China.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, PR China.

出版信息

Vet Microbiol. 2020 Jan;240:108508. doi: 10.1016/j.vetmic.2019.108508. Epub 2019 Nov 13.

DOI:10.1016/j.vetmic.2019.108508

PMID:31902493

Abstract

Duck Tembusu virus (DTMUV) is a major pathogen of duck industry in China. In the current study, we generated different constructs containing envelope (E) protein, pre-membrane-envelope (prM-E) protein, and C-terminally truncated E protein of the DTMUV. The constructed proteins could induce specific antibody responses in young ducks. When ducklings were immunized with the constructed proteins, they were 100% protected against DTMUV infection. Furthermore, the fluorescent signal of the truncated E protein was stronger than other constructed proteins, when Bac-to-Bac baculovirus expression system was applied. Our data demonstrated that the truncated E protein used in the current study could be applied as a potential vaccine candidate to control DTMUV infection in young ducks.

摘要

鸭坦布苏病毒（DTMUV）是中国养鸭业的主要病原体。在本研究中，我们构建了包含 E 蛋白、前膜-包膜（prM-E）蛋白和 DTMUV C 末端截短 E 蛋白的不同构建体。这些构建的蛋白可以在小鸭中诱导特异性抗体反应。当雏鸭用这些构建的蛋白免疫时，它们可以 100%免受 DTMUV 感染。此外，当应用 Bac-to-Bac 杆状病毒表达系统时，截短 E 蛋白的荧光信号强于其他构建的蛋白。我们的数据表明，本研究中使用的截短 E 蛋白可作为一种潜在的疫苗候选物，用于控制小鸭中的 DTMUV 感染。