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利用双光子荧光探针实现活细胞和组织中甾体硫酸酯酶的比率成像。

Achieving the ratiometric imaging of steroid sulfatase in living cells and tissues with a two-photon fluorescent probe.

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.

出版信息

Chem Commun (Camb). 2020 Jan 30;56(9):1349-1352. doi: 10.1039/c9cc08672b.

DOI:10.1039/c9cc08672b
PMID:31904042
Abstract

Herein, a novel two-photon ratiometric fluorescence assay was proposed for monitoring endogenous steroid sulfatase (STS) activity, which could be applied for the ratiometric imaging of STS activity in the endoplasmic reticulum of living cells and tissues and also could be used to distinguish estrogen-dependent tumor cells from other types of cells.

摘要

本文提出了一种新型的双光子比率荧光测定法，用于监测内源性甾体硫酸酯酶 (STS) 的活性，该方法可用于活细胞和组织内质网中 STS 活性的比率成像，也可用于区分雌激素依赖性肿瘤细胞与其他类型的细胞。

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