Huang Jiaqi, Jia Ru, Wei Xiaojing, Luo Xiao
Department of Physiology and Pathophysiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Dec 30;39(12):1494-1499. doi: 10.12122/j.issn.1673-4254.2019.12.15.
To investigate the time-sequential expression of a novel long non-coding RNA, lnc AK079912, in metabolically related tissues and during adipose tissue development and browning in mice.
The interscapular brown adipose tissue (iBAT), subcutaneous white adipose tissue (sWAT), epididymal white adipose tissue (eWAT), liver tissues and muscular tissues were collected from 8-week-old C57BL/6J mice. The iBAT, sWAT and eWAT were also collected from the mice during development (0 day, 21 days, 8 weeks and 6 months after birth) and from 8- to 10-week- mice with cold exposure (4 ℃) and intraperitoneal injections of CL316, 243 (1 μg/g body weight) for 1 to 5 days. Trizol was used to extract the total RNA from the tissues, and RT-qPCR was performed to detect the expressions of lnc AK079912. Isolated mouse preadipocytes in primary culture were induced for adipogenic differentiation for 9 days and then treated with CL316, 243 (2 μmol/L) for different durations (no longer than 24 h); the expression of lnc AK079912 in the cells was detected using RT-qPCR at different time points of the treatment.
Lnc AK079912 was highly expressed in mouse adipose tissues, the highest in iBAT, followed by the muscular tissue, but was hardly detected in the liver tissue. The expression level of lnc AK079912 increased progressively in iBAT and sWAT during development of the mice, while its expression in eWAT showed an initial increase followed by a reduction at 8 weeks ( < 0.001). No significant difference was found in the expression of lnc AK079912 in the iBAT, sWAT or eWAT in mice with cold stimulation for 1 to 5 days ( > 0.05). The expression of lnc AK079912 was significantly decreased in iBAT and eWAT ( < 0.05) but increased in eWAT from mice with intraperitoneal injection of CL316, 243 for 1 to 5 days ( < 0.05). The expression level in the adipocytes in primary culture was significantly increased in response to treatment with CL316, 243 ( < 0.05).
Lnc AK079912 is highly expressed in mouse adipose tissue, and its expression gradually increases with the development of adipose tissue but with a depot-specific difference. Lnc AK079912 is significantly elevated in the early stage of adipose tissue browning, indicating its important role in the development and browning of adipose tissue.
研究一种新型长链非编码RNA（lnc AK079912）在小鼠代谢相关组织以及脂肪组织发育和褐变过程中的时序表达。
从8周龄的C57BL/6J小鼠中收集肩胛间棕色脂肪组织（iBAT）、皮下白色脂肪组织（sWAT）、附睾白色脂肪组织（eWAT）、肝脏组织和肌肉组织。在小鼠发育过程中（出生后0天、21天、8周和6个月）以及对8至10周龄小鼠进行冷暴露（4℃）并腹腔注射CL316,243（1μg/g体重）1至5天，收集iBAT、sWAT和eWAT。用Trizol从组织中提取总RNA，并进行RT-qPCR检测lnc AK079912的表达。将原代培养的分离小鼠前脂肪细胞诱导成脂分化9天，然后用CL316,243（2μmol/L）处理不同时间（不超过24小时）；在处理的不同时间点用RT-qPCR检测细胞中lnc AK079912的表达。
lnc AK079912在小鼠脂肪组织中高表达，在iBAT中最高，其次是肌肉组织，而在肝脏组织中几乎未检测到。在小鼠发育过程中，lnc AK079912在iBAT和sWAT中的表达水平逐渐升高，而其在eWAT中的表达在8周时先升高后降低（P＜＜0.001）。冷刺激1至5天的小鼠iBAT、sWAT或eWAT中lnc AK079912的表达无显著差异（P＞0.05）。腹腔注射CL316,243 1至5天的小鼠iBAT和eWAT中lnc AK079912的表达显著降低（P＜0.05），而eWAT中lnc AK079912的表达升高（P＜0.05）。原代培养的脂肪细胞中，CL316,243处理后表达水平显著升高（P＜0.05）。
lnc AK079912在小鼠脂肪组织中高表达，其表达随脂肪组织发育逐渐增加，但存在部位特异性差异。lnc AK079912在脂肪组织褐变早期显著升高，表明其在脂肪组织发育和褐变中起重要作用。
