Luo Xiao, Jia Ru, Zhang Qiangling, Sun Bo, Yan Jianqun
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an 710061, China.
Int J Mol Sci. 2016 May 23;17(5):795. doi: 10.3390/ijms17050795.
Cold exposure or β₃-adrenoceptor agonist treatment induces the adipose tissues remodeling, relevant for beige adipogenesis within white adipose tissue (WAT). It remains unclear whether this process influences inflammatory adipokines expression in adipose tissues. We determine the temporal profile of cold or β₃-adrenoceptor agonist (CL316,243)-induced changes in the expression of inflammatory adipokines in adipose tissues in mice or primary mice adipocytes. Male C57BL/6J mice at eight weeks old were exposed to 4 °C for 1-5 days. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous WAT (sWAT) and epididymal WAT (eWAT) were harvested for gene and protein expression analysis. In addition, cultured primary mice brown adipocyte (BA) and white adipocyte (WA) treated with or without CL316,243 were harvested for gene expression analysis. The inflammatory adipokines expressed significantly higher in WAT than BAT at baseline. They were rapidly changed in iBAT, while down-regulated in sWAT and up-regulated in eWAT during the cold acclimation. Upon CL316,243 treatment, detected inflammatory adipokines except Leptin were transiently increased in both BA and WA. Our in vivo and in vitro data demonstrate that the browning process alters the inflammatory adipokines expression in adipose tissues, which is acutely responded to in iBAT, dynamically decreased in sWAT whilst increased in eWAT for compensation.
冷暴露或β₃-肾上腺素能受体激动剂治疗可诱导脂肪组织重塑，这与白色脂肪组织（WAT）中的米色脂肪生成有关。目前尚不清楚该过程是否会影响脂肪组织中炎性脂肪因子的表达。我们确定了寒冷或β₃-肾上腺素能受体激动剂（CL316,243）诱导的小鼠或原代小鼠脂肪细胞脂肪组织中炎性脂肪因子表达变化的时间特征。8周龄的雄性C57BL/6J小鼠暴露于4℃ 1-5天。采集肩胛间棕色脂肪组织（iBAT）、腹股沟皮下WAT（sWAT）和附睾WAT（eWAT）用于基因和蛋白质表达分析。此外，收集用或不用CL316,243处理的原代小鼠棕色脂肪细胞（BA）和白色脂肪细胞（WA）用于基因表达分析。在基线时，炎性脂肪因子在WAT中的表达明显高于BAT。在冷适应过程中，它们在iBAT中迅速变化，而在sWAT中下调，在eWAT中上调。用CL316,243处理后，除瘦素外，检测到的炎性脂肪因子在BA和WA中均短暂增加。我们的体内和体外数据表明，褐变过程改变了脂肪组织中炎性脂肪因子的表达，iBAT对此有急性反应，sWAT中动态降低，而eWAT中升高以进行补偿。
