Tange Yoshihiro, Yoshitake Shigenori, Watanabe Wataru
Department of Medical Engineering, Kyushu University of Health and Welfare, Nobeoka City, Japan.
Data Brief. 2019 Dec 17;28:105011. doi: 10.1016/j.dib.2019.105011. eCollection 2020 Feb.
Nitric oxide (NO) is a vasodilator and platelet aggregation inhibitor. In patients with pulmonary hypertension, inhalation of NO is used as a therapeutic option. It has been proposed that nitrite (NO ) is a constitute intravascular storage and delivery source of NO, a potent cardioprotective-signaling molecule. The administration of NO could have therapeutic effects in conditions where the oxygen-dependent enzymatic production of NO is compromised (i.e., ischemia). Thus, if NO could be supplied by an intravenous infusion fluid, it would be an easier method than by inhalation or delivery to the blood vessels by the blood stream. We produced 2 types of solutions, i.e., a nitrogen gas injected solution (control solution) and NO gas injected solution (experimental solution). NO was measured by the Microplate Photometer (MultiSkan FC, Thermo Fisher Scientific K.K., Tokyo, Japan) with a 540-nm wavelength and NO assay kit (Quantichrom™ Nitric Oxide Assay Kit, BioAssay Systems, Hayward, CA, USA). Gas profiles were measured by the EG6+ (Abbott Japan Co., Ltd., Osaka, Japan) with an i-STAT system (300F, Abbott Japan Co., Ltd.). Comparisons of gas profiles and measured NO concentrations in vitro and ex vivo are shown between the control and experimental solutions. Since NO is oxidized to NO and nitrate (NO ), it is common practice to quantitate total NO /NO as a measure of the NO level. We used the assay that was designed to accurately measure NO production following reduction of NO to NO using the Griess method. The data in this document describe production of an infusion fluid that contains NO without any special devices.
一氧化氮（NO）是一种血管扩张剂和血小板聚集抑制剂。在肺动脉高压患者中，吸入NO被用作一种治疗选择。有人提出，亚硝酸盐（NO₂⁻）是NO的一种血管内储存和释放来源，NO是一种强大的心脏保护信号分子。在NO的氧依赖性酶促生成受损的情况下（即缺血），给予NO₂⁻可能具有治疗作用。因此，如果可以通过静脉输液来提供NO，那将是一种比吸入或通过血流输送到血管更容易的方法。我们制备了两种溶液，即注入氮气的溶液（对照溶液）和注入NO气体的溶液（实验溶液）。使用波长为540 nm的微孔板光度计（MultiSkan FC，赛默飞世尔科技日本公司，东京，日本）和NO检测试剂盒（Quantichrom™一氧化氮检测试剂盒，BioAssay Systems，美国加利福尼亚州海沃德）测量NO。使用带有i-STAT系统（300F，雅培日本有限公司）的EG6 +（雅培日本有限公司，大阪，日本）测量气体成分。展示了对照溶液和实验溶液在体外和离体状态下气体成分和测量的NO浓度的比较。由于NO会被氧化为NO₂⁻和硝酸盐（NO₃⁻），通常将总NO₂⁻/NO₃⁻定量作为NO水平的一种度量。我们使用了一种检测方法，该方法旨在通过格里斯方法将NO₂⁻还原为NO后准确测量NO的生成。本文档中的数据描述了一种无需任何特殊装置即可产生含NO的输液的方法。
