宽叶缬草精油的细胞毒性活性。

Cytotoxic Activity of Cunila angustifolia Essential Oil.

机构信息

Natural Products Research Group, Federal University of Rio Grande, St. Cel. Francisco Borges de Lima, n. 3005, Bom Princípio, Santo Antônio da Patrulha, 95500-000, RS, Brazil.

Federal University of Santa Catarina, St. Eng. Agronômico Andrei Cristian Ferreira, Trindade, Florianópolis, 88040-900, SC, Brazil.

出版信息

Chem Biodivers. 2020 Feb;17(2):e1900656. doi: 10.1002/cbdv.201900656. Epub 2020 Jan 27.

DOI:10.1002/cbdv.201900656

PMID:31910318

Abstract

The Cunila angustifolia essential oil was obtained from fresh leaves by hydrodistillation and analyzed by GC-FID and GC-MS to determine its chemical composition. The essential oil presented pulegone (29.5 %) and isomenthol (27.0 %) as major components, and other compounds such as menthone (8.6 %), neomenthol (7.2 %), menthyl acetate (2.5 %) and caryophyllene oxide (2.0 %) were identified. The cytotoxic activity of the essential oil was evaluated by MTS assay, with the human cancer cell lines of the lung (A549), breast (MCF-7) and skin melanoma (SK-Mel-28). The assay showed the highest selectivity, to MCF-7 cell lines, with IC equal to 34.0 μg mL , low selectivity for SK-Mel-28 cell lines, with IC equal to 279.9 μg mL , and no mortality to A549 cell lines.

摘要

狭叶山香圆精油采用水蒸气蒸馏法从新鲜叶片中提取，并用 GC-FID 和 GC-MS 进行分析，以确定其化学成分。该精油的主要成分是薄荷酮（29.5%）和异胡薄荷醇（27.0%），还鉴定出其他化合物，如薄荷醇（8.6%）、新薄荷醇（7.2%）、乙酸薄荷酯（2.5%）和石竹烯氧化物（2.0%）。采用 MTS 法评价了精油对人肺癌（A549）、乳腺癌（MCF-7）和皮肤黑色素瘤（SK-Mel-28）细胞系的细胞毒性。结果表明，该精油对 MCF-7 细胞系具有最高的选择性，IC 为 34.0 μg/mL，对 SK-Mel-28 细胞系的选择性较低，IC 为 279.9 μg/mL，对 A549 细胞系无致死性。