Postgraduate Program in Natural Products and Bioactive Synthetics, Federal University of Paraíba, João Pessoa 58051-970, PB, Brazil.
Laboratory of Risk Assessment for Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraíba, João Pessoa 58051-970, PB, Brazil.
Biomolecules. 2023 Sep 24;13(10):1439. doi: 10.3390/biom13101439.
The essential oil from (Asteraceae) aerial parts (CBEO) was extracted by hydrodistillation in a Clevenger-type apparatus and was characterized by gas chromatography-mass spectrometry. The antitumor potential was evaluated against human tumor cell lines (melanoma, cervical, colorectal, and leukemias), as well as non-tumor keratinocyte lines using the MTT assay. The effect of CBEO on the production of Reactive Oxygen Species (ROS) was evaluated by DCFH-DA assay, and a protection assay using the antioxidant N-acetyl-L-cysteine (NAC) was also performed. Moreover, the CBEO toxicity in the zebrafish model was assessed. The majority of the CBEO compound was ()-2-lachnophyllum ester (57.24%). The CBEO exhibited selectivity towards SK-MEL-28 melanoma cells (half maximal inhibitory concentration, IC = 18.65 ± 1.16 µg/mL), and induced a significant increase in ROS production. In addition, the CBEO's cytotoxicity against SK-MEL-28 cells was reduced after pretreatment with NAC. Furthermore, after 96 h of exposure, 1.5 µg/mL CBEO induced death of all zebrafish embryos. Non-lethal effects were observed after exposure to 0.50-1.25 µg/mL CBEO. Additionally, significant alterations in the activity of enzymes associated with oxidative stress in zebrafish larvae were observed. These results provide evidence that CBEO has a significant in vitro antimelanoma effect by increasing ROS production and moderate embryotoxicity in zebrafish.
（菊科）地上部分的精油（CBEO）通过 Clevenger 型仪器的水蒸馏法提取，并通过气相色谱-质谱法进行了表征。采用 MTT 法评估了其对人肿瘤细胞系（黑色素瘤、宫颈、结直肠和白血病）以及非肿瘤角质形成细胞系的抗肿瘤潜力。通过 DCFH-DA 测定法评估了 CBEO 对活性氧 (ROS) 产生的影响，并使用抗氧化剂 N-乙酰-L-半胱氨酸 (NAC) 进行了保护测定。此外，还评估了 CBEO 在斑马鱼模型中的毒性。CBEO 中的大多数化合物为 ()-2-lachnophyllum 酯（57.24%）。CBEO 对 SK-MEL-28 黑色素瘤细胞具有选择性（半最大抑制浓度，IC = 18.65 ± 1.16 µg/mL），并诱导 ROS 产生显著增加。此外，在用 NAC 预处理后，CBEO 对 SK-MEL-28 细胞的细胞毒性降低。此外，暴露 96 小时后，1.5 µg/mL 的 CBEO 诱导所有斑马鱼胚胎死亡。暴露于 0.50-1.25 µg/mL 的 CBEO 后观察到非致死性影响。此外，还观察到斑马鱼幼虫中与氧化应激相关的酶活性发生了显著改变。这些结果表明，CBEO 通过增加 ROS 产生和适度的胚胎毒性在斑马鱼中具有显著的体外抗黑色素瘤作用。
