Xu Ci, Zhang Meichao, Bian Lei, Li Yanyan, Yao Yuan, Li Dong
Department of Radiation Oncology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Oncogenesis. 2020 Jan 8;9(1):4. doi: 10.1038/s41389-019-0188-1.
SGK196 is a protein O-mannose kinase involved in an indispensable phosphorylation step during laminin-binding glycan synthesis on alpha-dystroglycan (α-DG). However, the function of SGK196 in cancer diseases remains elusive. In the current study, we demonstrated that SGK196 is primarily modified by N-glycosylation in breast cancer (BC) cells. Furthermore, gain and loss-of-function studies showed that N-glycosylated SGK196 suppresses cell migration, invasion, and metastasis in BC, particularly in the basal-like breast cancer (BLBC) type. In addition, we found that SGK196 N-glycosylation performs the regulatory function through the PI3K/AKT/GSK3β signaling pathway. Collectively, our results show that N-glycosylated SGK196 plays suppression roles in BLBC metastases, therefore providing new insights into SGK196 function in BC.
SGK196是一种蛋白质O-甘露糖激酶，参与α- dystroglycan（α-DG）上层粘连蛋白结合聚糖合成过程中不可或缺的磷酸化步骤。然而，SGK196在癌症疾病中的功能仍不清楚。在本研究中，我们证明SGK196在乳腺癌（BC）细胞中主要通过N-糖基化修饰。此外，功能获得和功能丧失研究表明，N-糖基化的SGK196抑制BC细胞的迁移、侵袭和转移，特别是在基底样乳腺癌（BLBC）类型中。此外，我们发现SGK196 N-糖基化通过PI3K/AKT/GSK3β信号通路发挥调节功能。总的来说，我们的结果表明N-糖基化的SGK196在BLBC转移中起抑制作用，因此为SGK196在BC中的功能提供了新的见解。
