Egge H, Kordowicz M, Peter-Katalinić J, Hanfland P
J Biol Chem. 1985 Apr 25;260(8):4927-35.
A triantennary ceramide pentadecasaccharide (BIrab-2) with blood group I and B-like activity and an unbranched ceramide heptasaccharide (Birab) with i- and B-like activity were isolated in high yield from rabbit erythrocyte membranes. The structures of the native substances and the products obtained after treatment with alpha-galactosidase (BIrab-2 alpha, Birab alpha) and subsequent Smith degradation (BIrab-2 alpha SD) were determined by sugar analysis, methylation analysis, and fast atom bombardment-mass spectrometry of the permethylated derivatives. Together with the results of 1H NMR analysis (Dabrowski, U., Hanfland, P., Egge, H., Kuhn, S., and Dabrowski, J. (1984) J. Biol. Chem. 259, 7649-7651), the following structures were established for the native substances: (formula; see text) and Gal alpha 1----3Gal beta 1----4GlcNAc beta 1----3Gal beta 1----4GlcNAc beta 1----3Gal beta 1----4Glc beta 1----1Cer. Both compounds exhibit strong blood group B-like activity. BIrab-2 alpha is a strong receptor for human anti-I cold agglutinin and Birab for anti-i cold agglutinin.
从兔红细胞膜中高产率地分离出具有血型I和B样活性的三分支神经酰胺十五糖（BIrab - 2）以及具有i和B样活性的无分支神经酰胺七糖（Birab）。通过糖分析、甲基化分析以及对全甲基化衍生物的快原子轰击质谱法，确定了天然物质以及用α - 半乳糖苷酶处理后（BIrab - 2α，Birabα）并随后进行史密斯降解（BIrab - 2αSD）所得到产物的结构。结合¹H NMR分析结果（达布罗夫斯基，U.，汉夫兰德，P.，埃格，H.，库恩，S.，以及达布罗夫斯基，J.（1984年）《生物化学杂志》259卷，7649 - 7651页），确定了天然物质的以下结构：（分子式；见正文）以及Galα1→3Galβ1→4GlcNAcβ1→3Galβ1→4GlcNAcβ1→3Galβ1→4Glcβ1→1Cer。这两种化合物都表现出很强的血型B样活性。BIrab - 2α是人类抗I冷凝集素的强受体，而Birab是抗i冷凝集素的强受体。
