Lu Jianfei, Shen Zhaoyuan, Lu Liqun, Xu Dan
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, China.
Front Microbiol. 2019 Dec 18;10:2923. doi: 10.3389/fmicb.2019.02923. eCollection 2019.
DNA viruses, most notably members of the herpesvirus family, generally encode miRNAs to mediate both virus and host genes expression. We previously demonstrated that Cyprinid herpesvirus 2 (CyHV-2) encodes 17 miRNAs that are involved in innate immune signaling pathways. In this study, the function of CyHV-2-encoded miRNA was further investigated in GiCF cells. We found that miR-C4 promoted CyHV-2-induced apoptosis, while miR-C12 decreased CyHV-2-induced apoptosis. miR-C12 targeted to 3' UTR sequence of and suppressed the expression of . Besides, the silencing of caspase 8 by specific siRNA led to the attenuation of CyHV-2-induced apoptosis. Furthermore, caspase 8 was downregulated in cells transfected with miR-C12 during CyHV-2 infection. Overexpression of miR-C12 significantly suppressed CyHV-2-induced apoptosis, while silencing of miR-C12 promoted CyHV-2-induced apoptosis. Finally, inhibition of miR-C12 resulted in suppression of CyHV-2 propagation, overexpression of miR-C12, and CASP8-siRNA-1 facilitated CyHV-2 propagation. Taken together, our results demonstrated that CyHV-2-encoded miR-C12 to suppress virus-induced apoptosis and promoted virus replication by targeting .
DNA病毒，最显著的是疱疹病毒科的成员，通常编码微小RNA（miRNA）来介导病毒和宿主基因的表达。我们之前证明鲤疱疹病毒2型（CyHV-2）编码17种参与先天免疫信号通路的miRNA。在本研究中，我们在GiCF细胞中进一步研究了CyHV-2编码的miRNA的功能。我们发现miR-C4促进CyHV-2诱导的细胞凋亡，而miR-C12减少CyHV-2诱导的细胞凋亡。miR-C12靶向……的3'非翻译区（UTR）序列并抑制……的表达。此外，用特异性小干扰RNA（siRNA）沉默半胱天冬酶8导致CyHV-2诱导的细胞凋亡减弱。此外，在CyHV-2感染期间，用miR-C12转染的细胞中半胱天冬酶8表达下调。miR-C12的过表达显著抑制CyHV-2诱导的细胞凋亡，而miR-C12的沉默促进CyHV-2诱导的细胞凋亡。最后，抑制miR-C12导致CyHV-2增殖受到抑制，miR-C12的过表达以及CASP8-siRNA-1促进CyHV-2增殖。综上所述，我们的结果表明，CyHV-2编码的miR-C12通过靶向……来抑制病毒诱导的细胞凋亡并促进病毒复制。 （注：原文中部分内容缺失，用“……”表示）
