Waltzek Thomas B, Kelley Garry O, Stone David M, Way Keith, Hanson Larry, Fukuda Hideo, Hirono Ikuo, Aoki Takashi, Davison Andrew J, Hedrick Ronald P
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
The Centre for the Environment, Fisheries and Aquaculture Science, Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
J Gen Virol. 2005 Jun;86(Pt 6):1659-1667. doi: 10.1099/vir.0.80982-0.
The sequences of four complete genes were analysed in order to determine the relatedness of koi herpesvirus (KHV) to three fish viruses in the family Herpesviridae: carp pox herpesvirus (Cyprinid herpesvirus 1, CyHV-1), haematopoietic necrosis herpesvirus of goldfish (Cyprinid herpesvirus 2, CyHV-2) and channel catfish virus (Ictalurid herpesvirus 1, IcHV-1). The genes were predicted to encode a helicase, an intercapsomeric triplex protein, the DNA polymerase and the major capsid protein. The results showed that KHV is related closely to CyHV-1 and CyHV-2, and that the three cyprinid viruses are related, albeit more distantly, to IcHV-1. Twelve KHV isolates from four diverse geographical areas yielded identical sequences for a region of the DNA polymerase gene. These findings, with previously published morphological and biological data, indicate that KHV should join the group of related lower-vertebrate viruses in the family Herpesviridae under the formal designation Cyprinid herpesvirus 3 (CyHV-3).
为了确定鲤疱疹病毒（KHV）与疱疹病毒科的三种鱼类病毒的亲缘关系，对四个完整基因的序列进行了分析：鲤痘疱疹病毒（鲤疱疹病毒1型，CyHV-1）、金鱼造血坏死疱疹病毒（鲤疱疹病毒2型，CyHV-2）和斑点叉尾鮰病毒（鮰疱疹病毒1型，IcHV-1）。预测这些基因编码一种解旋酶、一种衣壳间三聚体蛋白、DNA聚合酶和主要衣壳蛋白。结果表明，KHV与CyHV-1和CyHV-2密切相关，并且这三种鲤科病毒与IcHV-1也有亲缘关系，尽管关系较远。来自四个不同地理区域的12株KHV分离株在DNA聚合酶基因的一个区域产生了相同的序列。这些发现与先前发表的形态学和生物学数据表明，KHV应在正式命名为鲤疱疹病毒3型（CyHV-3）的情况下加入疱疹病毒科中相关的低等脊椎动物病毒组。
