Paradiso Francesca, Fitzgerald Joan, Yao Seydou, Barry Frank, Taraballi Francesca, Gonzalez Deyarina, Conlan R Steven, Francis Lewis
Reproductive Biology and Gynaecological Oncology Group, Swansea University Medical School, Swansea, United Kingdom.
Center for Biomimetic Medicine, Houston Methodist Research Institute, Houston, TX, United States.
Front Bioeng Biotechnol. 2019 Dec 13;7:343. doi: 10.3389/fbioe.2019.00343. eCollection 2019.
A fundamental structural component of extracellular matrix in all connective and interstitial tissue, collagen is the most abundant protein in the human body. To date, mammalian collagens sources represent the golden standard for multiple biomedical applications, while marine-derived collagens have largely been used in industry (food, pharmaceutical, and cosmetic), with little use in research and clinical applications. Herein we demonstrate the effective use jellyfish collagen, a source of biocompatible, sustainable collagen for 2D and 3D cell culture, addressing the global drive for technological developments that result in the replacement of animals and their derived products in research. Jellyfish collagen harbors similar structural features mammalian collagen type I, despite differing slightly in amino acid content. Jellyfish collagen supports ovarian cancer (OvCa) cell line proliferation, cellular morphology and expression of epithelial to mesenchymal transition markers, supporting the use of as a non-mammalian collagen cell culture substrate. Furthermore, collagen is effective in 3D device fabrication such as sponges where it mimics tissue architecture complexity. OvCa cells migrated and differentiated within the collagen 3D scaffolds confirming its suitability for advanced cell culturing applications, providing an excellent alternative to mammalian collagen sources for the culture of human cells.
胶原蛋白是所有结缔组织和间质组织细胞外基质的基本结构成分，是人体中含量最丰富的蛋白质。迄今为止，哺乳动物来源的胶原蛋白是多种生物医学应用的黄金标准，而海洋来源的胶原蛋白主要用于工业（食品、制药和化妆品），在研究和临床应用中使用较少。在此，我们展示了海蜇胶原蛋白的有效用途，这是一种用于二维和三维细胞培养的生物相容性、可持续的胶原蛋白来源，满足了全球对技术发展的需求，即实现在研究中替代动物及其衍生产品。海蜇胶原蛋白具有与哺乳动物I型胶原蛋白相似的结构特征，尽管氨基酸含量略有不同。海蜇胶原蛋白支持卵巢癌细胞系增殖、细胞形态以及上皮-间质转化标志物的表达，支持将其用作非哺乳动物胶原蛋白细胞培养底物。此外，海蜇胶原蛋白在三维装置制造（如海绵）中有效，它能模拟组织结构的复杂性。卵巢癌细胞在海蜇胶原蛋白三维支架内迁移和分化，证实了其适用于先进的细胞培养应用，为人类细胞培养提供了一种替代哺乳动物胶原蛋白来源的绝佳选择。
