Department of ICU, Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
Nursing Department, Minhang District Maternal and Child Health Hospital of Shanghai Minhang, Shanghai 201102, P.R. China.
Mol Med Rep. 2020 Mar;21(3):1304-1309. doi: 10.3892/mmr.2020.10928. Epub 2020 Jan 10.
Long noncoding (lnc)RNA HAND2‑AS1 inhibits the development of several human malignancies. The role of HAND2‑AS1 was investigated in hepatocellular carcinoma (HCC). It was found that levels of HAND2‑AS1 in serum were significantly lower, while serum levels of Rho‑associated protein kinase 2 (ROCK2) in HCC patients were significantly increased compared with hepatitis B (HB) patients and healthy controls. Decreased HAND2‑AS1 levels distinguished HCC patients but not HB patients from healthy controls. A significant negative correlation between HAND2‑AS1 and ROCK2 was found in HCC patients but not in HB patients or healthy controls. HAND2‑AS1 overexpression inhibited, while ROCK2 overexpression promoted HCC cell migration, proliferation and invasion. HAND2‑AS1 overexpression led to downregulated ROCK2 expression. ROCK2 overexpression did not significantly affect ROCK2 expression but attenuated the inhibitory effects of HAND2‑AS1 overexpression. It was therefore concluded that HAND2‑AS1 might mediate the downregulation of ROCK2 in HCC to inhibit cancer cell migration, proliferation and invasion.
长链非编码 RNA HAND2-AS1 抑制多种人类恶性肿瘤的发展。研究了 HAND2-AS1 在肝细胞癌 (HCC) 中的作用。结果发现，与乙型肝炎 (HB) 患者和健康对照组相比，HCC 患者血清 HAND2-AS1 水平显著降低，而 Rho 相关蛋白激酶 2 (ROCK2) 血清水平显著升高。与 HB 患者相比，HAND2-AS1 水平降低可区分 HCC 患者和健康对照组。在 HCC 患者中发现 HAND2-AS1 与 ROCK2 呈显著负相关，但在 HB 患者或健康对照组中未发现。HAND2-AS1 过表达抑制 HCC 细胞迁移、增殖和侵袭，而 ROCK2 过表达促进 HCC 细胞迁移、增殖和侵袭。HAND2-AS1 过表达导致 ROCK2 表达下调。ROCK2 过表达对 ROCK2 表达没有显著影响，但减弱了 HAND2-AS1 过表达的抑制作用。因此，HAND2-AS1 可能通过下调 HCC 中的 ROCK2 来抑制癌细胞迁移、增殖和侵袭。
