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荔枝废弃物衍生的碳点:在实际水样中检测 Fe 离子和皮肤黑色素瘤细胞的多色细胞成像中的应用。

Carbon dots derived from lychee waste: Application for Fe ions sensing in real water and multicolor cell imaging of skin melanoma cells.

机构信息

Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.

Department of Zoology, University of Kalyani, Kalyani, 741235, West Bengal, India.

出版信息

Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110429. doi: 10.1016/j.msec.2019.110429. Epub 2019 Nov 14.

Abstract

Exploit of biomass as an inexhaustible resource has accepted much more curiosity to the present research world. Herein, a simple, one-step solvothermal action has been used to synthesize an ascendable amount of fluorescent carbon dots (CDs) with an average size of~3.13 nm, from Low-reasonable and green source lychee waste. The excitation/emission maxima of CDs have 365/443 nm with high quantum yield (23.5%). The present ingredient predominantly contained carboxylic acid and hydroxyl group that acted as a passive agent for stabilizing the CDs. The structural and optical properties were evaluated through HRTEM, FTIR, UV-vis, zeta potential, XPS, fluorescence, and fluorescence lifetime experiments. We investigated the manoeuvre of our synthesized CDs as a probe for detection of Fe ions in water bodies; This sensing approach showed impressive selectivity and sensitivity towards Feions with LOD 23.6 nM. The sensing mechanism took place through static quenching which was entrenched through fluorescence lifetime measurements. Fe ions detection was basically carried out with efficacy in real water. For its lofty Photo-stability, low cytotoxicity and cell viability the probe were substantially applied for bio-imaging experiment i.e. intracellular multi-color cell imaging in skin melanoma cells (A375 cells) with and without Fe ions exemplifying its real applications in living cells.

摘要

生物质作为一种取之不尽的资源,已经引起了当今研究界的极大兴趣。在此,我们采用了一种简单的一步溶剂热法,从低合理性和绿色来源荔枝废物中合成了大量具有平均尺寸约为 3.13nm 的荧光碳点(CDs)。CDs 的激发/发射最大值为 365/443nm,量子产率为 23.5%。目前的成分主要含有羧基和羟基,它们作为稳定 CD 的被动剂。通过 HRTEM、FTIR、UV-vis、zeta 电位、XPS、荧光和荧光寿命实验对结构和光学性质进行了评估。我们研究了我们合成的 CDs 作为水体中 Fe 离子检测探针的作用;这种传感方法对 Fe 离子表现出令人印象深刻的选择性和灵敏度,LOD 为 23.6nM。传感机制通过荧光寿命测量证实了通过静态猝灭发生。Fe 离子的检测基本上可以在实际水中有效进行。由于其高的光稳定性、低细胞毒性和细胞活力,探针被广泛应用于生物成像实验,即在皮肤黑色素瘤细胞(A375 细胞)中进行细胞内多色细胞成像,有和没有 Fe 离子,证明了其在活细胞中的实际应用。

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