Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, V.le J.F. Kennedy 54, 80125, Naples, Italy.
Institute for Polymers, Composites and Biomaterials, National Research Council of Italy, Mostra d'Oltremare, V.le J.F. Kennedy 54, 80125, Naples, Italy.
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110385. doi: 10.1016/j.msec.2019.110385. Epub 2019 Nov 5.
The growing interest in the use of polysaccharides nanoparticles for biomedical applications is related to the recent progresses on the synthesis of cellulose-based polymers with the specific functionalities. In particular, cellulose graft copolymers are emerging as amphiphilic materials suitable to fabricate smart nanoparticles for drug delivery applications. In this work, a cellulose-graft-poly(ε-caprolactone) (cell-g-PCL) was synthetized and characterized by FTIR, TGA and DSC in order to validate the synthesis process. We demonstrated that fast evaporation processes promoted cell-g-PCL self-assembly to form nanomicellar structures with hydrodynamic radius ranged from 30 to 60 nm as confirmed by TEM analysis. Moreover, the application of controlled electrostatic forces on solvent evaporation - namely electrospraying - allowed generating round-like nanoscaled particles, as confirmed by SEM supported via image analysis. We demonstrated also that sodium diclofenac (DS) drastically influenced the mechanism of particle formation, favoring the deposition of erythrocyte-like particles with highly concave surfaces, not penalizing the encapsulation efficiency of nanoparticles (>80%). The release profile showed a fast delivery of DS - about 60% during the first 24 h - followed by a sustained release - about 20% during the next 6 days - strictly related to the peculiar weak interactions among amphiphilic polymer segments and water molecules, thus suggesting a successful use of electrosprayed cell-g-PCL nanoparticles for therapeutic treatments in nanomedicine.
人们对多糖纳米粒子在生物医学应用中的兴趣日益浓厚，这与最近在合成具有特定功能的纤维素基聚合物方面的进展有关。特别是，纤维素接枝共聚物作为适用于药物输送应用的两亲性材料，正在崭露头角。在这项工作中，合成了纤维素接枝聚（ε-己内酯）（cell-g-PCL），并通过 FTIR、TGA 和 DSC 进行了表征，以验证合成过程。我们证明了快速蒸发过程促进了 cell-g-PCL 的自组装，形成了水动力半径在 30 至 60nm 之间的纳米胶束结构，这一点通过 TEM 分析得到了证实。此外，通过控制溶剂蒸发过程中的静电力 - 即电喷雾 - 生成了类似圆形的纳米级颗粒，这一点通过 SEM 分析得到了证实，并通过图像分析进行了支持。我们还证明，双氯芬酸钠（DS）极大地影响了颗粒形成的机制，有利于沉积具有高度凹陷表面的红细胞样颗粒，而不会降低纳米颗粒的包封效率（>80%）。释放曲线显示 DS 的快速释放 - 在前 24 小时内约为 60% - 随后是持续释放 - 在下一个 6 天内约为 20% - 这与两亲聚合物段与水分子之间的特殊弱相互作用密切相关，因此表明电喷雾 cell-g-PCL 纳米粒子在纳米医学中的治疗应用中具有成功的应用前景。
