Matthew Maurice A, Christie Jevan, Yang Nawu, Yao Chaoqun
Department of Biomedical Sciences, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre 00334, Saint Kitts and Nevis.
One Health Centre for Zoonosis and Tropical Veterinary Diseases, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre 00334, Saint Kitts and Nevis.
Microorganisms. 2022 Mar 10;10(3):594. doi: 10.3390/microorganisms10030594.
is a flagellated protozoan that inhabits the human and canine oral cavity in patients with poor oral hygiene and periodontal disease. The loop-mediated isothermal amplification (LAMP) assay could provide clinicians with a quick, cheap and reliable diagnostic test used for the detection of in various settings. In this study, we aimed to develop a LAMP assay that can detect with high sensitivity and specificity. A set of LAMP primers were specifically designed to detect the ITS and 5.8S rRNA gene of . The newly developed LAMP assay was 1000 times more sensitive than conventional PCR. The limit of detection of the LAMP assay was 10 fg of genomic DNA, or 0.2-1 cell. Moreover, the LAMP assay was specific, resulting in no cross-reaction even with a closely related protozoan or other microorganisms (, , , , and ) used. The present LAMP assay can be performed directly without prior DNA extraction, making the assay an easy, fast, cheap, specific and sensitive diagnostic tool for the detection of at the point-of-care of both medical and veterinary clinics in developed and developing countries.
是一种有鞭毛的原生动物，在口腔卫生差和患有牙周病的人类和犬类口腔中生存。环介导等温扩增（LAMP）检测法可为临床医生提供一种快速、廉价且可靠的诊断检测方法，用于在各种环境中检测。在本研究中，我们旨在开发一种能以高灵敏度和特异性检测的LAMP检测法。设计了一组LAMP引物，专门用于检测的ITS和5.8S rRNA基因。新开发的LAMP检测法比传统PCR灵敏1000倍。LAMP检测法的检测限为10 fg基因组DNA，或0.2 - 1个细胞。此外，LAMP检测法具有特异性，即使与密切相关的原生动物或其他微生物（、、、、和）一起使用也不会产生交叉反应。目前的LAMP检测法无需事先提取DNA即可直接进行，这使得该检测法成为一种在发达国家和发展中国家的医疗和兽医诊所即时护理点检测的简便、快速、廉价、特异且灵敏的诊断工具。
