一项利用普通狨猴（狨猴）建立人类1型T细胞白血病病毒（HTLV-1）携带者模型的初步研究。
A pilot study to establish human T-cell leukemia virus type 1 (HTLV-1) carrier model using common marmoset (Callithrix jacchus).
作者信息
Hirose Lisa, Hiramoto Takafumi, Tian Yamin, Kohara Hiroshi, Kobayashi Seiichiro, Nagai Etsuko, Denda Tamami, Tanaka Yukihisa, Ota Yasunori, Jiyuan Liao, Miyamoto Shohei, Miura Yoshie, Hijikata Yasuki, Soda Yasushi, Inoue Takashi, Okahara Norio, Itoh Toshio, Sasaki Erika, Tojo Arinobu, Uchimaru Kaoru, Tani Kenzaburo
机构信息
Project Division of ALA Advanced Medical Research, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Division of Molecular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
出版信息
J Med Primatol. 2020 Apr;49(2):86-94. doi: 10.1111/jmp.12454. Epub 2020 Jan 12.
BACKGROUND
For the diagnosis and treatment of adult T-cell leukemia/lymphoma (ATLL) caused by human T-lymphotropic virus type 1 (HTLV-1) are required therapeutic modalities urgently. Non-human primate models for ATLL would provide a valuable information for clinical studies. We did a pilot study to establish an ATLL non-human primate model using common marmosets (Callithrix jacchus).
METHODS
We inoculated HTLV-1-producing MT-2 cells into 9-month-old marmosets, either intraperitoneally or intravenously. We next administrated MT-2 cells into 13-month-old marmosets under cyclosporine A (CsA) treatment to promote infection. HTLV-1 infection was determined by measuring HTLV-1 antibody titer in the common marmosets.
RESULTS
The HTLV-1 antibody titer increased in the intraperitoneally inoculated marmoset with or without CsA treatment, and it kept over five 5 years though proviral copy number (proviral load, PVL) remained low throughout the study.
CONCLUSION
We obtained HTLV-1 asymptomatic carriers of common marmosets by inoculating MT-2 cells.
背景
对于由1型人类嗜T淋巴细胞病毒（HTLV-1）引起的成人T细胞白血病/淋巴瘤（ATLL），急需有效的治疗方法。ATLL的非人灵长类动物模型将为临床研究提供有价值的信息。我们进行了一项初步研究，以使用普通狨猴（Callithrix jacchus）建立ATLL非人灵长类动物模型。
方法
我们将产生HTLV-1的MT-2细胞腹腔内或静脉内接种到9个月大的狨猴中。接下来，我们在环孢素A（CsA）治疗下将MT-2细胞接种到13个月大的狨猴中，以促进感染。通过测量普通狨猴中的HTLV-1抗体滴度来确定HTLV-1感染。
结果
在接受或未接受CsA治疗的腹腔内接种的狨猴中，HTLV-1抗体滴度均升高，并且在整个研究过程中，尽管前病毒拷贝数（前病毒载量，PVL）一直很低，但该抗体滴度在5年多的时间里一直保持升高。
结论
通过接种MT-2细胞，我们获得了普通狨猴的HTLV-1无症状携带者。
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