Xu Daogun, Wang Wei, Bian Tun, Yang Weifang, Shao Minghai, Yang Haihua
Laboratory of Cellular and Molecular Radiation Oncology, Affiliated Taizhou Hospital of Wenzhou Medical University Taizhou, Zhejiang Province, China.
Department of Colorectal Surgery in Wenling Traditional Chinese Medicine Hospital Wenling, Taizhou, Zhejiang Province, China.
Int J Clin Exp Pathol. 2019 Apr 1;12(4):1305-1314. eCollection 2019.
Recent studies suggest that Elevated O-GlcNAcylation by increased O-GlcNAc transferase (OGT) and/or decreasing O-GlcNAcase (OGA) levels is associated with cancer initiation, progression, invasion, and metastasis. However, the function of OGT in colon cancer tumorigeneses remains unclear. Here, we showed OGT expression is elevated in human colon cancer tissue compared with adjacent normal tissue, and cases with higher OGT expression had shorter survival time. Additionally, OGT mRNA expression was positively correlated with pathologic TNM stage from TCGA public database. Finally, we found knock-down of OGT expression by RNA interference inhibits cell proliferation, migration and invasion in colon cancer cell lines. Taken together, this study imply that elevated OGT expression had an important function in colon cancer formation and progression, and OGT may be a valuable prognostic factor and therapeutic target.
最近的研究表明，通过增加O-连接N-乙酰葡糖胺转移酶（OGT）和/或降低O-连接N-乙酰葡糖胺酶（OGA）水平而导致的O-连接N-乙酰葡糖胺糖基化升高与癌症的发生、发展、侵袭和转移有关。然而，OGT在结肠癌肿瘤发生中的作用仍不清楚。在此，我们发现与相邻正常组织相比，OGT在人结肠癌组织中的表达升高，且OGT表达较高的病例生存时间较短。此外，从TCGA公共数据库可知，OGT mRNA表达与病理TNM分期呈正相关。最后，我们发现通过RNA干扰敲低OGT表达可抑制结肠癌细胞系中的细胞增殖、迁移和侵袭。综上所述，本研究表明OGT表达升高在结肠癌的形成和发展中具有重要作用，且OGT可能是一个有价值的预后因素和治疗靶点。
