微小RNA-107靶向TRIAP1以调控口腔鳞状细胞癌的增殖和迁移。
miR-107 targets TRIAP1 to regulate oral squamous cell carcinoma proliferation and migration.
作者信息
Na Cunjirigala, Li Xiaojing, Zhang Jinghui, Han Lijuan, Li Ying, Zhang Hui
机构信息
Department of Stomatology, Inner Mongolia Baogang Hospital (The Third Affiliated Hospital of Inner Mongolia Medical University) Baotou, Inner Mongolia, P. R. China.
出版信息
Int J Clin Exp Pathol. 2019 May 1;12(5):1820-1825. eCollection 2019.
The role of microRNA-107 (miR-107) as a tumor suppressor has been explored in different types of human cancer. However, the expression and role of miR-107 in oral squamous cell carcinoma (OSCC) remains elusive. Here, the expression of miR-107 in OSCC cell lines was explored by reverse transcription-quantitative polymerase chain reaction. Online prediction algorithms and luciferase activity reporter assay were conducted to validate the targets of miR-107. Cell counting kit-8 assay and wound-healing assay were performed to analyze the functions of miR-107 on OSCC cells. These results indicated that miR-107 had reduced expression in OSCC cells. Overexpression of miR-107 inhibits OSCC cell proliferation and migration. It was found that miR-107 directly targets TP53 regulated inhibitor of apoptosis 1 (TRIAP1) to regulate gene expression. Together, these results demonstrated that miR-107 also plays a tumor suppressive role by inhibiting proliferation and migration of OSCC cells by targeting TRIAP1. Our finding provides novel insights into the mechanism of OSCC progression.
微小RNA-107（miR-107）作为一种肿瘤抑制因子在不同类型的人类癌症中的作用已得到研究。然而，miR-107在口腔鳞状细胞癌（OSCC）中的表达及作用仍不清楚。在此，通过逆转录-定量聚合酶链反应探究了miR-107在OSCC细胞系中的表达。利用在线预测算法和荧光素酶活性报告基因检测来验证miR-107的靶标。采用细胞计数试剂盒-8检测法和伤口愈合检测法分析miR-107对OSCC细胞的功能。这些结果表明，miR-107在OSCC细胞中的表达降低。miR-107的过表达抑制OSCC细胞的增殖和迁移。研究发现，miR-107直接靶向TP53调控的凋亡抑制因子1（TRIAP1）以调节基因表达。总之，这些结果表明，miR-107还通过靶向TRIAP1抑制OSCC细胞的增殖和迁移而发挥肿瘤抑制作用。我们的发现为OSCC进展机制提供了新的见解。
Zotero 插件