Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Laboratory of Photobiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Nutrients. 2020 Jan 9;12(1):180. doi: 10.3390/nu12010180.
The natural carotenoid crocetin has been reported to suppress phenotypes of an experimental myopia model in mice. We investigated the minimum effective dose to prevent myopia progression in a murine model. Three-week-old male mice (C57B6/J) were equipped with a -30 diopter (D) lens to induce myopia, and fed with normal chow, 0.0003%, or 0.001% of crocetin-containing chow. Changes in refractive errors and axial lengths (AL) were evaluated after three weeks. Pharmacokinetics of crocetin in the plasma and the eyeballs of mice was evaluated with specific high sensitivity quantitative analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine the minimum effective dosage. A concentration of 0.001% of crocetin-containing chow showed a significant ( < 0.001) suppressive effect against both refractive and AL changes in the murine model. Meanwhile, there was no significant difference of AL change between the 0.0003% and the normal chow groups. The concentration of crocetin in the plasma and the eyeballs from mice fed with 0.001% crocetin-containing chow was significantly higher than control and 0.0003% crocetin-containing chow. In conclusion, we suggest 0.001% of crocetin-containing extract is the minimum effective dose showing a significant suppressive effect against both refractive and AL changes in the murine model.
天然类胡萝卜素藏红花酸已被报道可抑制实验性近视模型中小鼠的表型。我们研究了预防小鼠模型中近视进展的最小有效剂量。将 3 周龄雄性小鼠（C57B6/J）配备-30 屈光度（D）镜片以诱导近视，并喂食正常饲料、0.0003%或 0.001%的含藏红花酸的饲料。三周后评估屈光度和眼轴（AL）的变化。使用特定的高灵敏度定量分析（液相色谱串联质谱法 [LC-MS/MS]）评估藏红花酸在小鼠血浆和眼球中的药代动力学，以确定最小有效剂量。0.001%的藏红花酸饲料显示出对小鼠模型的屈光度和 AL 变化有显著（<0.001）的抑制作用。同时，0.0003%的藏红花酸饲料与正常饲料组之间的 AL 变化没有显著差异。从喂食 0.001%藏红花酸饲料的小鼠的血浆和眼球中藏红花酸的浓度明显高于对照和 0.0003%藏红花酸饲料组。综上所述，我们建议 0.001%的含藏红花酸提取物是显示对小鼠模型的屈光度和 AL 变化有显著抑制作用的最小有效剂量。
