Functional Foods Forum, Faculty of Medicine, University of Turku, Itäinen Pitkäkatu 4A, 20520 Turku, Finland.
DuPont Nutrition and Biosciences, Sokeritehtaantie 20, 02460 Kantvik, Finland.
Nutrients. 2020 Jan 9;12(1):182. doi: 10.3390/nu12010182.
Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.
早产并发症是五岁以下儿童死亡的主要原因。预防策略包括使用巴氏消毒的捐赠人乳（DHM）、用人乳强化剂（蛋白质补充剂）强化，以及补充益生菌。我们的目的是研究 DHM 和强化 DHM（FDHM）对两种广泛使用的益生菌的黏液黏附特性的影响。该研究涵盖了两种形式的人乳强化剂，液体和粉末，有无益生菌，并在 4°C 下储存 24 小时。为了测试益生菌菌株的黏附特性，制备了 DHM+益生菌和 FDHM+益生菌，并添加到从健康芬兰婴儿粪便中分离出的固定化黏液中。然后通过液体闪烁法测量益生菌的黏附。我们的结果表明，在捐赠人乳中添加液体或粉末人乳强化剂对益生菌的黏附没有影响。此外，鉴于缓冲液中悬浮的益生菌黏附增加，还需要进一步研究其他基质。在为早产儿设计使用益生菌的未来干预策略时，需要考虑这些因素。
