Mantziari Anastasia, Mannila Enni, Collado Maria Carmen, Salminen Seppo, Gómez-Gallego Carlos
Functional Foods Forum, Faculty of Medicine, University of Turku, 20520 Turku, Finland.
Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), 46980 Valencia, Spain.
Microorganisms. 2021 Jun 7;9(6):1239. doi: 10.3390/microorganisms9061239.
Adhesion to intestinal mucus is the first step for microbiota colonization in early life. Polyamines are polycations with important physiological functions in both procaryotic and eucaryotic cells. However, their role in intestinal mucus adhesion is not known. The objective of the present study was to evaluate whether exogenous polyamines (putrescine, spermidine, spermine, and their combination) would alter the adhesive properties of GG (LGG), subs. Bb12, , and . Human intestinal mucus was isolated from healthy infants (0-6-month-old and 6-12-month-old) and healthy adults (25-52 years old). Spermidine significantly increased Bb12 adhesion ( < 0.05) in the mucus of infants (0-6 months) but reduced the adhesion of LGG in adult mucus ( < 0.05) with no significant effect in any of the infant groups. Spermine was more effective than polyamine combinations in reducing ( < 0.05) adhesion in early infant mucus (0-6 months). The adhesion ability of remained unaffected by exogenous polyamines at any age in the concentrations tested. Our data suggest that polyamines may modulate the bacterial adhesion to mucus depending on the bacterial strain and depending at what age the mucus has been generated.
黏附于肠道黏液是微生物群在生命早期定殖的第一步。多胺是多阳离子，在原核细胞和真核细胞中都具有重要的生理功能。然而，它们在肠道黏液黏附中的作用尚不清楚。本研究的目的是评估外源性多胺（腐胺、亚精胺、精胺及其组合）是否会改变嗜酸乳杆菌GG（LGG）、嗜双歧杆菌亚种Bb12等菌株的黏附特性。从健康婴儿（0至6个月和6至12个月）和健康成年人（25至52岁）中分离出人类肠道黏液。亚精胺显著增加了婴儿（0至6个月）黏液中Bb12的黏附（P<0.05），但降低了LGG在成人黏液中的黏附（P<0.05），对任何婴儿组均无显著影响。在减少早期婴儿（0至6个月）黏液中嗜酸乳杆菌的黏附方面，精胺比多胺组合更有效（P<0.05）。在所测试的浓度下，嗜酸乳杆菌的黏附能力在任何年龄均不受外源性多胺的影响。我们的数据表明，多胺可能根据细菌菌株以及黏液产生的年龄来调节细菌对黏液的黏附。
