Yanagimoto Keita, Yamagami Takaya, Uematsu Kosei, Haramoto Eiji
Department of Microbiology, Yamanashi Institute of Public Health and Environment, 1-7-31 Fujimi, Kofu, Yamanashi 400-0027, Japan.
Environmental and Social System Science Course, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
Pathogens. 2020 Jan 10;9(1):52. doi: 10.3390/pathogens9010052.
is a major cause of gastroenteritis usually caused by animal-based contaminated foods. Since the current passive surveillance is not sufficient to detect all infections and infection sources, we determined the prevalence of isolated from sewage influent of wastewater treatment plants (WWTPs) and compared the characteristics of human and food isolates to identify the infection sources. Sewage influent samples were collected monthly from two WWTPs located in the Yamanashi Prefecture, Japan, for three years. Serotypes, antimicrobial resistances, isolation periods, isolated areas, and pulsed-field gel electrophoresis patterns of six isolates belonging to five serotypes were consistent with those of the isolates from patients. Real-time PCR for indicated that sewage influents reflect cases of patients infected with , including unreported cases. Serovars Schwarzengrund and Anatum were predominant in sewage, but not in humans, and their characteristics were closely related or identical to those isolated from poultry heart and liver, respectively. These results suggest that sewage influent contains isolates from humans and that some originated from unreported human cases infected by poultry-associated products. Therefore, it is necessary to take countermeasures against infection based on the unreported cases, which would be disclosed by analysis of sewage influent.
是肠胃炎的主要病因，通常由动物性污染食品引起。由于当前的被动监测不足以检测到所有感染和感染源，我们确定了从污水处理厂（WWTPs）进水污水中分离出的[病原体名称未给出]的流行情况，并比较了人类和食品分离株的特征以确定感染源。在三年时间里，每月从日本山梨县的两个污水处理厂采集进水污水样本。属于五种血清型的六个分离株的血清型、抗菌药物耐药性、分离时期、分离地区和脉冲场凝胶电泳图谱与患者分离株一致。针对[病原体名称未给出]的实时PCR表明，进水污水反映了感染[病原体名称未给出]的患者病例，包括未报告的病例。施瓦岑格鲁德血清型和阿纳托姆血清型在污水中占主导，但在人类中并非如此，它们的特征分别与从家禽心脏和肝脏分离出的菌株密切相关或相同。这些结果表明，进水污水中含有来自人类的[病原体名称未给出]分离株，并且一些源于未报告的由家禽相关产品感染的人类病例。因此，有必要根据未报告的病例采取针对[病原体名称未给出]感染的对策，这些病例将通过进水污水分析得以揭示。
