人体肠道定植 crAss 样噬菌体的获取、传播和株系多样性。
Acquisition, transmission and strain diversity of human gut-colonizing crAss-like phages.
机构信息
Department of Genetics, Stanford University, Stanford, CA, USA.
Department of Medicine, Division of Hematology, Stanford University, Stanford, CA, USA.
出版信息
Nat Commun. 2020 Jan 15;11(1):280. doi: 10.1038/s41467-019-14103-3.
CrAss-like phages are double-stranded DNA viruses that are prevalent in human gut microbiomes. Here, we analyze gut metagenomic data from mother-infant pairs and patients undergoing fecal microbiota transplantation to evaluate the patterns of acquisition, transmission and strain diversity of crAss-like phages. We find that crAss-like phages are rarely detected at birth but are increasingly prevalent in the infant microbiome after one month of life. We observe nearly identical genomes in 50% of cases where the same crAss-like clade is detected in both the mother and the infant, suggesting vertical transmission. In cases of putative transmission of prototypical crAssphage (p-crAssphage), we find that a subset of strains present in the mother are detected in the infant, and that strain diversity in infants increases with time. Putative tail fiber proteins are enriched for nonsynonymous strain variation compared to other genes, suggesting a potential evolutionary benefit to maintaining strain diversity in specific genes. Finally, we show that p-crAssphage can be acquired through fecal microbiota transplantation.
类 CrAss 噬菌体是一种双链 DNA 病毒，普遍存在于人类肠道微生物组中。在这里，我们分析了母婴对和接受粪便微生物群移植的患者的肠道宏基因组数据，以评估类 CrAss 噬菌体的获得、传播和菌株多样性模式。我们发现，类 CrAss 噬菌体在出生时很少被检测到，但在生命的第一个月后，在婴儿微生物组中越来越普遍。我们观察到在 50%的情况下，母亲和婴儿中都检测到相同的类 CrAss 进化枝，这表明存在垂直传播。在假定的原型 crAssphage（p-crAssphage）传播的情况下，我们发现母亲中存在的一些菌株在婴儿中被检测到，并且婴儿中的菌株多样性随着时间的推移而增加。与其他基因相比，假定的尾部纤维蛋白富含非同义菌株变异，这表明在特定基因中维持菌株多样性具有潜在的进化优势。最后，我们表明 p-crAssphage 可以通过粪便微生物群移植获得。
Zotero 插件