crAssphage与腹泻无关，且具有高度的遗传多样性。
crAssphage is not associated with diarrhoea and has high genetic diversity.
作者信息
Liang Y Y, Zhang W, Tong Y G, Chen S P
机构信息
Department of Laboratory Medicine,Affiliated Hospital of Academy of Military Medical Sciences,Beijing,PR China.
State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing,PR China.
出版信息
Epidemiol Infect. 2016 Dec;144(16):3549-3553. doi: 10.1017/S095026881600176X.
crAssphage is a newly discovered gut bacteriophage. However, its pathogenicity and molecular epidemiology in humans are as yet unclear. In this study, we investigated the association between crAssphage and diarrhoea, as well as the molecular epidemiology of crAssphage in Chinese patients from our hospital. Our results indicated that there were no significant differences in the crAssphage-positive ratio and viral loads in faecal supernatants between adults with diarrhoea and healthy adults. Of infants and children with diarrhoea, 2·8% were found to be crAssphage-positive, including two infants aged <1 month. Markedly, of all confirmed crAssphage-positive strains, 100% had the ORF00039 deletion and 77·8% had low identity of ORF00018 compared to crAssphage (GenBank accession no. NC_024711, designated genotype 1). Thus, crAssphage was not associated with diarrhoea and most strains of crAssphage in Chinese patients (designated genotype 2) were characterized by the ORF00039 deletion and low identity of ORF00018.
crAssphage是一种新发现的肠道噬菌体。然而，其在人类中的致病性和分子流行病学尚不清楚。在本研究中，我们调查了crAssphage与腹泻之间的关联，以及我院中国患者中crAssphage的分子流行病学。我们的结果表明，腹泻成人与健康成人粪便上清液中crAssphage阳性率和病毒载量无显著差异。在腹泻婴幼儿中，发现2.8%为crAssphage阳性，其中包括两名年龄小于1个月的婴儿。值得注意的是，在所有确诊的crAssphage阳性菌株中，100%存在ORF00039缺失，与crAssphage（GenBank登录号NC_024711，指定为基因型1）相比，77.8%的ORF00018同源性较低。因此，crAssphage与腹泻无关，中国患者中大多数crAssphage菌株（指定为基因型2）的特征是ORF00039缺失和ORF00018同源性较低。
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