Sharaf M M, Nestor K E, Saif Y M, Sacco R E, Havenstein G B
Department of Poultry Science, Ohio State University, Wooster 44691.
Poult Sci. 1988 Oct;67(10):1372-7. doi: 10.3382/ps.0671372.
The primary and secondary response to Newcastle disease virus (NCDV) and Pasteurella multocida (PM) of two turkey lines were studied following vaccinations with either NCDV or PM alone, or in combination. The two turkey strains were 1) a randombred control line (RBC1) and 2) a subline (E) of Line RBC1 selected 27 generations for increased egg production. This study consisted of five trials. Each trial represented a separate hatch. In Trial 1, poults of each line were subcutaneously vaccinated with a 1-mL dilution of B1 type LaSota strain NCDV vaccine. In Trial 2, poults of each line were wing-web vaccinated with the M-9 strain of PM Heddleston Type 3 x 4 cross at 6 and 10 wk of age. In Trial 3, poults of each line were subcutaneously vaccinated on the back of the neck with 1 mL of inactivated NCDV at 6 and 10 wk of age. In Trial 4, .5 mL of a PM bacterin containing Types 1, 3, and 4 in an oil emulsion was used to subcutaneously vaccinate poults of each line at 6 and 10 wk of age. In Trial 5, poults of each line were simultaneously vaccinated with inactivated NCDV (subcutaneously) and a PM bacterin (intramuscularly) at 6 and 10 wk of age. Line RBC1 had significantly (P less than .01) higher maternal antibodies to Newcastle disease at 3 wk of age than those of Line E. The RBC1 line generally had significantly higher levels of antibodies than Line E in response to vaccination for both NCDV and PM when administered singularly or in combination.
研究了两个火鸡品系分别单独接种新城疫病毒（NCDV）或多杀性巴氏杆菌（PM），以及联合接种这两种疫苗后，对NCDV和PM的初次和二次应答。这两个火鸡品系分别为：1）一个随机交配对照品系（RBC1），以及2）RBC1品系中为提高产蛋量而选择了27代的一个亚系（E）。本研究包括五项试验。每项试验代表一个单独的孵化批次。在试验1中，给每个品系的雏火鸡皮下接种1 mL稀释的B1型LaSota株NCDV疫苗。在试验2中，给每个品系的雏火鸡在6周龄和10周龄时通过翅蹼接种M-9株Heddleston 3型×4型交叉的PM疫苗。在试验3中，给每个品系的雏火鸡在6周龄和10周龄时于颈部后方皮下接种1 mL灭活NCDV。在试验4中，使用0.5 mL含1、3和4型的油乳剂PM菌苗，在6周龄和10周龄时给每个品系的雏火鸡皮下接种。在试验5中，给每个品系的雏火鸡在6周龄和10周龄时同时皮下接种灭活NCDV和肌肉注射PM菌苗。RBC1品系在3周龄时对新城疫的母源抗体显著（P小于0.01）高于E品系。当单独或联合接种NCDV和PM疫苗时，RBC1品系在接种疫苗后的抗体水平通常显著高于E品系。
