Department of Obstetrics and Gynecology, Breast Center, University Hospital, LMU Munich, 80337 Munich, Germany.
Department of Pathology, LMU Munich, 80337 Munich, Germany.
Int J Mol Sci. 2020 Jan 3;21(1):330. doi: 10.3390/ijms21010330.
The aim of this study was to investigate the expression of thyroid hormone receptor β1 (THRβ1) by immunohistochemistry in breast cancer (BC) tissues and to correlate the results with clinico-biological parameters. In a well-characterized cohort of 274 primary BC patients, THRβ1 was widely expressed with a predominant nuclear location, although cytoplasmic staining was also frequently observed. Both nuclear and cytoplasmic THRβ1 were correlated with high-risk BC markers such as human epidermal growth factor receptor 2 (HER2), Ki67 (also known as MKI67), prominin-1 (CD133), and N-cadherin. Overall survival analysis demonstrated that cytoplasmic THRβ1 was correlated with favourable survival ( = 0.015), whereas nuclear THRβ1 had a statistically significant correlation with poor outcome ( = 0.038). Interestingly, in our cohort, nuclear and cytoplasmic THRβ1 appeared to be independent markers either for poor ( = 0.0004) or for good ( = 0.048) prognosis, respectively. Altogether, these data indicate that the subcellular expression of THRβ1 may play an important role in oncogenesis. Moreover, the expression of nuclear THRβ1 is a negative outcome marker, which may help to identify high-risk BC subgroups.
本研究旨在通过免疫组织化学方法检测甲状腺激素受体 β1（THRβ1）在乳腺癌（BC）组织中的表达，并将结果与临床生物学参数相关联。在一个经过充分特征描述的 274 例原发性 BC 患者队列中，THRβ1 广泛表达，主要定位于核内，但也经常观察到细胞质染色。核内和细胞质 THRβ1 均与高风险 BC 标志物如人表皮生长因子受体 2（HER2）、Ki67（也称为 MKI67）、prominin-1（CD133）和 N-钙黏蛋白相关。总生存分析表明，细胞质 THRβ1 与良好的生存相关（=0.015），而核 THRβ1 与不良预后具有统计学显著相关性（=0.038）。有趣的是，在我们的队列中，核内和细胞质 THRβ1 似乎分别是不良（=0.0004）或良好（=0.048）预后的独立标志物。总之，这些数据表明 THRβ1 的亚细胞表达可能在肿瘤发生中发挥重要作用。此外，核 THRβ1 的表达是一种不良预后的标志物，可帮助识别高风险 BC 亚组。
