胰蛋白酶抑制剂重链4与卵巢癌之间的关系。
Relationship between inter--trypsin inhibitor heavy chain 4 and ovarian cancer.
作者信息
Huang Min, Zhang Wei, Zhao Bingbing, Li Li
机构信息
Department of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Department of Gynecologic and Obstetrics, The First Affiliated Hospital of Guilin Medical College, Guilin 541100, China.
出版信息
Chin J Cancer Res. 2019 Dec;31(6):955-964. doi: 10.21147/j.issn.1000-9604.2019.06.11.
OBJECTIVE
The inter-α-trypsin inhibitor heavy chain 4 (ITIH4) protein is involved in the development of tumors. However, the relationship between ITIH4 and ovarian cancer (OC) has not been extensively examined. This study aimed to explore the effect of ITIH4 on OC and to identify its underlying mechanism.
METHODS
Expressions of ITIH4 in OC tissues and cells were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blots. The function of ITIH4 in the OC cell line HO8910pm was tested via ITIH4 knockdown. The cell growth rate was measured using MTT and colony formation assays. Flow cytometry was performed to evaluate cell cycle progression. Cell migration and invasion abilities were observed using the transwell migration assay.
RESULTS
ITIH4 was downregulated in OC tissues and cells. ITIH4 knockdown promoted cell growth and cell cycle progression. Consistent with these results, inhibition of ITIH4 in OC cells significantly increased cell migration and invasion abilities. Cox regression analysis suggests that ITIH4 expression alone is not a good predictor of the prognosis of malignant ovarian tumors in patients.
CONCLUSIONS
ITIH4 inhibits the progression of OC, suggesting that ITIH4 may be a useful biomarker for OC. This study may provide a potential novel target for the treatment of OC.
目的
α-胰蛋白酶抑制剂重链4（ITIH4）蛋白参与肿瘤的发生发展。然而，ITIH4与卵巢癌（OC）之间的关系尚未得到广泛研究。本研究旨在探讨ITIH4对OC的影响并确定其潜在机制。
方法
采用定量逆转录聚合酶链反应（RT-qPCR）和蛋白质印迹法检测OC组织和细胞中ITIH4的表达。通过敲低ITIH4来检测其在OC细胞系HO8910pm中的功能。使用MTT和集落形成试验测量细胞生长速率。进行流式细胞术以评估细胞周期进程。使用Transwell迁移试验观察细胞迁移和侵袭能力。
结果
ITIH4在OC组织和细胞中表达下调。敲低ITIH4可促进细胞生长和细胞周期进程。与这些结果一致，抑制OC细胞中的ITIH4可显著提高细胞迁移和侵袭能力。Cox回归分析表明，仅ITIH4表达不是恶性卵巢肿瘤患者预后的良好预测指标。
结论
ITIH4抑制OC的进展，提示ITIH4可能是OC的有用生物标志物。本研究可能为OC治疗提供潜在的新靶点。
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