作为喉癌诊断的生物标志物。
acts as a biomarker for diagnosis of laryngeal cancer.
作者信息
Zhang Yi, Wang Zhiping, Bai Xiuqing, Xu Yaosheng
机构信息
Department of Otorhinolaryngological, Harrison International Peace Hospital Hengshui 053000, China.
出版信息
Int J Clin Exp Pathol. 2018 Oct 1;11(10):4996-5001. eCollection 2018.
BACKGROUND
Laryngeal cancer is a common malignancy of head and neck cancer, with increasing morbidity and mortality in our country. It is reported that can act as a serum marker for several diseases and cancers. In this study, we evaluated the diagnostic value of in laryngeal cancer.
METHODS
The serum levels of 119 laryngeal carcinoma patients and 68 healthy volunteers were detected by real-time PCR (qRT-PCR) and ELISA. Chi-square test was applied to evaluate the association between mRNA levels and clinical characteristics. To determine the diagnostic value of , ROC curve was constructed.
RESULTS
The expression level of was higher in laryngeal cancer patients than that in healthy group (<0.001). Moreover, its mRNA level was significantly associated with tumor size (=0.004), TNM stage (=0.006), and distant metastasis (=0.018). ROC curve demonstrated that could be used for laryngeal cancer diagnosis with AUC value of 0.924, combing with the sensitivity of 78.8% and specificity of 89.7%. The optimal cutoff value was 0.725.
CONCLUSION
is up-regulated and correlated with tumor progression in laryngeal cancer patients. may be a potential diagnostic biomarker for laryngeal cancer.
背景
喉癌是头颈部常见的恶性肿瘤，在我国其发病率和死亡率呈上升趋势。据报道， 可作为多种疾病和癌症的血清标志物。在本研究中，我们评估了 在喉癌中的诊断价值。
方法
采用实时荧光定量聚合酶链反应（qRT-PCR）和酶联免疫吸附测定（ELISA）检测119例喉癌患者和68例健康志愿者的血清 水平。应用卡方检验评估 信使核糖核酸（mRNA）水平与临床特征之间的关联。为确定 的诊断价值，构建了受试者工作特征（ROC）曲线。
结果
喉癌患者中 的表达水平高于健康组（<0.001）。此外，其mRNA水平与肿瘤大小（=0.004）、肿瘤-淋巴结-转移（TNM）分期（=0.006）及远处转移（=0.018）显著相关。ROC曲线显示， 可用于喉癌诊断，曲线下面积（AUC）值为0.924，敏感性为78.8%，特异性为89.7%。最佳截断值为0.725。
结论
在喉癌患者中上调且与肿瘤进展相关。 可能是喉癌潜在的诊断生物标志物。
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