Mougin B, Bakouche O, Gerlier D
INSERM U51, UA CNRS no. 613, Lyon, France.
Vaccine. 1988 Oct;6(5):445-9. doi: 10.1016/0264-410x(88)90147-8.
The immunogenicity of measles virus glycoproteins presented associated to liposomes or ISCOMs was compared with that of whole virus and solubilized membrane proteins in W/Fu rats. The rats were immunized three times at ten-day intervals with syngeneic peritoneal exudate cells fed in vitro with the various antigen preparations. A strong and persisting antibody response with haemagglutinin inhibitory and neutralization activity was observed in rats immunized with liposomes, ISCOMs, or virus. The responses were very similar despite the lower dose of protein received by rats immunized with ISCOMs (1 microgram protein) or with liposomes (20 micrograms protein). By contrast, injection of peritoneal exudate cells previously fed in vitro with soluble H and F glycoproteins resulted in only a poor and transient response. The sera from rats immunized with virus, liposomes or ISCOMs contained antibodies immunoprecipitating mainly H and F glycoproteins. Despite a strong enrichment in F polypeptides during the preparation of ISCOMs, they induced an equal anti-H and anti-F antibody response.
在W/Fu大鼠中，比较了与脂质体或免疫刺激复合物（ISCOMs）结合呈现的麻疹病毒糖蛋白的免疫原性与完整病毒及溶解的膜蛋白的免疫原性。用体外喂食了各种抗原制剂的同基因腹膜渗出细胞，以十天的间隔对大鼠进行三次免疫。在用脂质体、ISCOMs或病毒免疫的大鼠中观察到了具有血凝抑制和中和活性的强烈且持久的抗体反应。尽管用ISCOMs免疫的大鼠（1微克蛋白）或用脂质体免疫的大鼠（20微克蛋白）接受的蛋白剂量较低，但反应非常相似。相比之下，注射先前在体外喂食了可溶性H和F糖蛋白的腹膜渗出细胞仅产生了微弱且短暂的反应。用病毒、脂质体或ISCOMs免疫的大鼠血清中含有主要免疫沉淀H和F糖蛋白的抗体。尽管在ISCOMs的制备过程中F多肽有很强的富集，但它们诱导的抗H和抗F抗体反应相同。
