Franz J, Hampl J, Stĕpánek J, Tesarík R
Veterinary Research Institute, Brno, Czech Republic.
Vet Med (Praha). 1996 Jul;41(7):213-8.
Dynamics and persistence of antibody responses to immunization and reimmunization of rabbits with BHV-1 ISCOMs or BHV-1 liposomes were investigated for 20 weeks and compared with those induced by a commercial vaccine against infectious bovine rhinotracheitis (IBR) or free BHV-1 protein subunits. Comparable levels of antibodies to BHV-1, determined by ELISA, were found throughout the observation period in rabbits treated with BHV-1 ISCOMs or the commercial vaccine. The immunogenicity of BHV-1 liposomes was higher than that of the free virus proteins, but lower than those of BHV-1 ISCOMs or the commercial vaccine. Virus neutralization test confirmed the best immunostimulatory effect of BHV-1 ISCOMs as far as the levels and the persistence of antibodies were concerned. The reactivities of the specific virus protein subunits with anti-BHV-1 antibodies elicited in rabbits immunized with BHV-1 ISCOMs or the commercial vaccine were compared by Western blotting. The patterns of the bands were similar, but some differences were found in their numbers and intensities.
对用牛疱疹病毒1型（BHV-1）免疫刺激复合物（ISCOMs）或BHV-1脂质体免疫和再次免疫的兔子，研究其抗体反应的动力学和持久性，为期20周，并与一种针对传染性牛鼻气管炎（IBR）的商业疫苗或游离BHV-1蛋白亚基诱导的抗体反应进行比较。在用BHV-1 ISCOMs或商业疫苗处理的兔子中，在整个观察期内通过ELISA测定发现，针对BHV-1的抗体水平相当。BHV-1脂质体的免疫原性高于游离病毒蛋白，但低于BHV-1 ISCOMs或商业疫苗。就抗体水平和持久性而言，病毒中和试验证实BHV-1 ISCOMs具有最佳的免疫刺激效果。通过蛋白质印迹法比较了用BHV-1 ISCOMs或商业疫苗免疫的兔子中特定病毒蛋白亚基与抗BHV-1抗体的反应性。条带模式相似，但在数量和强度上发现了一些差异。
