Laboratory of Neurobiology of Inflammatory and Metabolic Processes, Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Av. José Acácio Moreira, 787, Tubarão, Santa Catarina, 88704-900, Brazil.
Laboratory of Experimental Pathophysiology, Postgraduate Program in Health Sciences, University of the Extreme South of Santa Catarina, Criciúma, Santa Catarina, Brazil.
Metab Brain Dis. 2024 Apr;39(4):635-648. doi: 10.1007/s11011-023-01311-6. Epub 2024 Mar 2.
Obesity results from an energy imbalance and has been considered an epidemic due to its increasing rates worldwide. It is classified as a low-grade chronic inflammatory disease and has associated comorbidities. Different nutritional strategies are used for the purpose of weight loss, highlighting low-carbohydrate (LC) diets, ketogenic diets, and intermittent fasting (IF). These strategies can lead to metabolic and behavioral changes as they stimulate different biochemical pathways. Therefore, this study evaluated memory, energy metabolism, neuroinflammation, oxidative stress, and antioxidant defense parameters in mice subjected to an LC diet, ketogenic diet (KD), or IF. Eighty male Swiss mice, 60 days old, were divided into 4 groups: control, LC, KD, or IF. Body weight was measured weekly, and food intake every 48 h. After 15 days of nutritional interventions, the animals were subjected to the behavioral object recognition test and subsequently euthanized. Then, visceral fat was removed and weighed, and the brain was isolated for inflammatory and biochemical analysis. We concluded from this study that the LC and KD strategies could damage memory, IF improves the production of adenosine triphosphate (ATP), and the LC, KD, and IF strategies do not lead to neuroinflammatory damage but present damage at the level of oxidative stress.
肥胖是由能量失衡引起的，由于其在全球范围内的发病率不断上升，已被视为一种流行病。它被归类为一种低度慢性炎症性疾病，并伴有相关的合并症。不同的营养策略被用于减肥，突出了低碳水化合物（LC）饮食、生酮饮食（KD）和间歇性禁食（IF）。这些策略可以通过刺激不同的生化途径导致代谢和行为的改变。因此，本研究评估了接受 LC 饮食、KD 或 IF 的小鼠的记忆、能量代谢、神经炎症、氧化应激和抗氧化防御参数。将 80 只 60 天大的雄性瑞士小鼠分为 4 组：对照组、LC 组、KD 组或 IF 组。每周测量体重，每 48 小时测量一次食物摄入量。在营养干预 15 天后，动物接受行为物体识别测试，然后处死。然后取出内脏脂肪并称重，并分离大脑进行炎症和生化分析。我们从这项研究中得出结论，LC 和 KD 策略可能会损害记忆，IF 会提高三磷酸腺苷（ATP）的产生，LC、KD 和 IF 策略不会导致神经炎症损伤，但会在氧化应激水平上造成损伤。
