NMR 观察蛋白质酪氨酸侧链 OH 与 DNA 磷酸基团之间的分子间氢键。
NMR Observation of Intermolecular Hydrogen Bonds between Protein Tyrosine Side-Chain OH and DNA Phosphate Groups.
机构信息
Department of Biochemistry & Molecular Biology, Sealy Center for Structural Biology & Molecular Biophysics , University of Texas Medical Branch , Galveston , Texas 77555-1068 , United States.
出版信息
J Phys Chem B. 2020 Feb 13;124(6):1065-1070. doi: 10.1021/acs.jpcb.9b10987. Epub 2020 Jan 30.
Abstract
Hydrogen bonds between protein side-chain hydroxyl (OH) and phosphate groups are one of the most common types of intermolecular hydrogen bonds in protein-DNA/RNA complexes. Using NMR spectroscopy, we identified and characterized the hydrogen bonds between tyrosine side-chain OH and DNA phosphate groups in a protein-DNA complex. These OH groups exhibited relatively slow hydrogen-exchange rates and sizable scalar couplings between hydroxyl H and DNA phosphate P nuclei across the hydrogen bonds. Information about intermolecular hydrogen bonds facilitates investigations of the DNA/RNA recognition by the protein.
摘要
蛋白质侧链羟基 (OH) 和磷酸基团之间的氢键是蛋白质-DNA/RNA 复合物中最常见的分子间氢键类型之一。我们使用 NMR 光谱学鉴定并描述了蛋白质-DNA 复合物中酪氨酸侧链 OH 和 DNA 磷酸基团之间的氢键。这些 OH 基团表现出相对较慢的氢交换率，并且在氢键两侧羟基 H 和 DNA 磷酸 P 核之间具有可观的标量耦合。有关分子间氢键的信息有助于研究蛋白质对 DNA/RNA 的识别。
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