通过横向弛豫优化光谱法观察到的DNA中跨越沃森-克里克碱基对氢键的核磁共振标量耦合。
NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
作者信息
Pervushin K, Ono A, Fernández C, Szyperski T, Kainosho M, Wüthrich K
机构信息
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule, Hönggerberg CH-8093 Zürich, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14147-51. doi: 10.1073/pnas.95.24.14147.
Abstract
This paper describes the NMR observation of 15N---15N and 1H---15N scalar couplings across the hydrogen bonds in Watson-Crick base pairs in a DNA duplex, hJNN and hJHN. These couplings represent new parameters of interest for both structural studies of DNA and theoretical investigations into the nature of the hydrogen bonds. Two dimensional [15N,1H]-transverse relaxation-optimized spectroscopy (TROSY) with a 15N-labeled 14-mer DNA duplex was used to measure hJNN, which is in the range 6-7 Hz, and the two-dimensional hJNN-correlation-[15N,1H]-TROSY experiment was used to correlate the chemical shifts of pairs of hydrogen bond-related 15N spins and to observe, for the first time, hJHN scalar couplings, with values in the range 2-3.6 Hz. TROSY-based studies of scalar couplings across hydrogen bonds should be applicable for large molecular sizes, including protein-bound nucleic acids.
摘要
本文描述了对DNA双链体中沃森-克里克碱基对间氢键上的15N---15N和1H---15N标量耦合的核磁共振观测，即hJNN和hJHN。这些耦合对于DNA结构研究和氢键本质的理论研究而言，都是新的重要参数。采用含15N标记的14聚体DNA双链体的二维[15N,1H]-横向弛豫优化光谱法（TROSY）来测量hJNN，其范围为6 - 7赫兹；二维hJNN相关-[15N,1H]-TROSY实验用于关联氢键相关的15N自旋对的化学位移，并首次观测到hJHN标量耦合，其值范围为2 - 3.6赫兹。基于TROSY对氢键上标量耦合的研究应适用于大分子尺寸，包括与蛋白质结合的核酸。
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