The Key Laboratory of Biomedical Materials, School of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003, People's Republic of China.
Biomater Sci. 2020 Mar 7;8(5):1431-1441. doi: 10.1039/c9bm01880h. Epub 2020 Jan 21.
Novel Janus nanoparticles (J-NPs) are developed by using single iron oxide (FeO) nanoparticles as the core and hydrophobic/hydrophilic polymeric brushes as the cloak. Because of the superparamagnetism and asymmetric functionality of J-NPs, they are used as drug carriers and therapeutic agents for cancer chemotherapy and magnetic hyperthermia with a magnetic resonance imaging (MRI) guide. The asymmetric functionality is constituted of hydrophobic polymethyl methacrylate (PMMA) brushes and hydrophilic polyacrylic acid (PAA) brushes, which are 'grafting to' or 'grafting from' FeO nanoparticles via activators regenerated by electron transfer atom transfer radical polymerization. The terminal chains of PMMA and PAA brushes are coordinated with FeO nanoparticles, so PMMA/FeO/PAA J-NPs possess structural stability in solvents. Because of the brush-structure, PMMA/FeO/PAA J-NPs show high encapsulation efficiency (89.75 ± 2.35%) and loading capacity (8.95 ± 0.26%). Under the alternating magnetic field (AMF), drug-loaded J-NPs achieve the highest cell proliferation-inhibition ratio in the cell proliferation test in vitro and the tumor growth inhibition test in vivo compared to single chemotherapy or magnetic hyperthermia. Meanwhile, J-NPs show good T imaging.
新型的双面纳米粒子（J-NPs）是通过使用单个氧化铁（FeO）纳米粒子作为核心，以及疏水性/亲水性聚合物刷作为外壳来开发的。由于 J-NPs 的超顺磁性和不对称功能，它们被用作药物载体和治疗癌症的化疗试剂，并在磁共振成像（MRI）引导下进行磁热疗。不对称功能由疏水性聚甲基丙烯酸甲酯（PMMA）刷和亲水性聚丙烯酸（PAA）刷组成，它们通过电子转移原子转移自由基聚合的再生剂“接枝到”或“从”FeO 纳米粒子上。PMMA 和 PAA 刷的末端链与 FeO 纳米粒子配位，因此 PMMA/FeO/PAA J-NPs 在溶剂中具有结构稳定性。由于刷状结构，PMMA/FeO/PAA J-NPs 表现出高的包封效率（89.75±2.35%）和载药量（8.95±0.26%）。在交变磁场（AMF）下，与单一化疗或磁热疗相比，载药的 J-NPs 在体外细胞增殖抑制试验和体内肿瘤生长抑制试验中表现出最高的细胞增殖抑制率。同时，J-NPs 表现出良好的 T 成像。
