Pasteurized protects from fat mass gain but not from bone loss.

Probiotic bacteria can protect from ovariectomy (ovx)-induced bone loss in mice. is considered to have probiotic potential due to its beneficial effect on obesity and insulin resistance. The purpose of the present study was to determine if treatment with pasteurized (p could prevent ovx-induced bone loss. Mice were treated with vehicle or p for 4 wk, starting 3 days before ovx or sham surgery. Treatment with p reduced fat mass accumulation confirming earlier findings. However, treatment with p decreased trabecular and cortical bone mass in femur and vertebra of gonadal intact mice and did not protect from ovx-induced bone loss. Treatment with p increased serum parathyroid hormone (PTH) levels and increased expression of the calcium transporter in kidney suggesting increased reabsorption of calcium in the kidneys. Serum amyloid A 3 (SAA3) can suppress bone formation and mediate the effects of PTH on bone resorption and bone loss in mice and treatment with p increased serum levels of SAA3 and gene expression of in colon. Moreover, regulatory T cells can be protective of bone and p-treated mice had decreased number of regulatory T cells in mesenteric lymph nodes and bone marrow. In conclusion, treatment with p protected from ovx-induced fat mass gain but not from bone loss and reduced bone mass in gonadal intact mice. Our findings with p differ from some probiotics that have been shown to protect bone mass, demonstrating that not all prebiotic and probiotic factors have the same effect on bone.