Caimi Christian, Gasco Laura, Biasato Ilaria, Malfatto Vanda, Varello Katia, Prearo Marino, Pastorino Paolo, Bona Maria Cristina, Francese Danila Raffaella, Schiavone Achille, Elia Antonia Concetta, Dörr Ambrosius Josef Martin, Gai Francesco
Department of Agricultural, Forest and Food Sciences, University of Torino, Largo P. Braccini 2, 10095 Grugliasco, Italy.
Veterinary Medical Research Institute for Piedmont, Liguria and Aosta Valley, Via Bologna 148, 10154 Torino, Italy.
Animals (Basel). 2020 Jan 16;10(1):155. doi: 10.3390/ani10010155.
The trial investigates if a highly defatted larva meal (H) at two dietary inclusion levels and a vegetable protein based diet (VEG) influences the normal gut and liver histology and the oxidative stress biomarkers in liver and kidney of Siberian sturgeon juveniles. Fish were fed four diets: one control diet (H0) containing 70% of fishmeal (FM), two diets including 18.5% (H185) and 37.5% (H375) of highly defatted H in substitution for 25% and 50% of FM, and one vegetable protein based diet (VEG). At the end of a growth trial, 12 fish per treatment were sacrificed by over-anaesthesia to collect 12 liver and 5 distal intestine samples for histological analyses, as well as 12 liver and kidney samples for biochemical analyses. The H and VEG diets did not significantly affect the histology of liver and distal intestine, but alterations of the oxidative stress biomarkers were detected at the highest inclusion level of H (37.5%). In order to avoid unfavorable effects on the fish health, an inclusion level up to 18.5% of H is recommended for Siberian sturgeon juveniles.
该试验研究了两种日粮添加水平的高度脱脂幼虫粉（H）和一种植物蛋白基日粮（VEG）是否会影响西伯利亚鲟幼鱼的正常肠道和肝脏组织学以及肝脏和肾脏中的氧化应激生物标志物。给鱼投喂四种日粮：一种对照日粮（H0），含有70%的鱼粉（FM）；两种日粮分别含有18.5%（H185）和37.5%（H375）的高度脱脂H，分别替代25%和50%的FM；以及一种植物蛋白基日粮（VEG）。在生长试验结束时，通过过度麻醉处死每个处理组的12条鱼，以收集12个肝脏和5个远端肠道样本用于组织学分析，以及12个肝脏和肾脏样本用于生化分析。H和VEG日粮对肝脏和远端肠道的组织学没有显著影响，但在H的最高添加水平（37.5%）下检测到氧化应激生物标志物的变化。为了避免对鱼的健康产生不利影响，建议西伯利亚鲟幼鱼的H添加水平最高为18.5%。
