Di Rosa Ambra Rita, Caccamo Letteria, Pansera Lidia, Oteri Marianna, Chiofalo Biagina, Maricchiolo Giulia
Department of Veterinary Sciences, University of Messina, Polo Annunziata, 98168 Messina, Italy.
Institute FOR MARINE Biological Resources and Biotechnology, National Research Council (IRBIM, CNR), Spianata S. Raineri 86, 98122 Messina, Italy.
Animals (Basel). 2023 Jan 18;13(3):339. doi: 10.3390/ani13030339.
This study provided new data and knowledge on the potential use of meal (HIM) as a new sustainable ingredient for diet. The effect of HIM dietary inclusion on fish growth performance, stress indicators and gut histology was studied. For 131 days, 312 fish were fed a basal diet containing fishmeal as animal protein source, and three diets containing 25%, 35% and 50% HIM as a partial replacement for fishmeal. The main findings indicated that fishmeal can be replaced by HIM up to 110 g/kg of substitution (35% of inclusion in diet) without negative effects on growth performance, stress parameters or histological traits of the posterior gut tract, and with positive effects ( < 0.05) on the histological and morphometric characteristics of the anterior gut tract. At the same time, the results showed that the effect of meal at 50% inclusion level caused morphometric and histopathological alterations in the anterior gut tract of seabream. In conclusion, this preliminary study suggested that the dietary inclusion level of HIM35 was the most tolerated by fish showing the best gut morphometric parameters and histological conditions, with fewer signs of inflammation, as well as good nutritional and health status.
本研究提供了关于食用昆虫粉（HIM）作为一种新的可持续饮食成分潜在用途的新数据和知识。研究了在饲料中添加HIM对鱼类生长性能、应激指标和肠道组织学的影响。在131天的时间里，给312条鱼投喂以鱼粉作为动物蛋白源的基础饲料，以及三种分别含有25%、35%和50% HIM作为鱼粉部分替代品的饲料。主要研究结果表明，鱼粉可以被HIM替代至110克/千克的替代量（占饲料的35%），而不会对生长性能、应激参数或后肠道的组织学特征产生负面影响，并且对前肠道的组织学和形态计量学特征有积极影响（P<0.05）。同时，结果显示，添加50%水平的HIM会导致海鲷前肠道的形态计量学和组织病理学改变。总之，这项初步研究表明，饲料中添加35%的HIM对鱼类的耐受性最强，表现出最佳的肠道形态计量学参数和组织学状况，炎症迹象较少，营养和健康状况良好。
