采用液相色谱-高分辨率质谱法（LC-HRMS）对蜂王浆游离脂肪酸进行研究。
Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS).
作者信息
Kokotou Maroula G, Mantzourani Christiana, Babaiti Rodalia, Kokotos George
机构信息
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece.
出版信息
Metabolites. 2020 Jan 16;10(1):40. doi: 10.3390/metabo10010040.
The lipidome of royal jelly (RJ) consists of medium-chained (8-12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection.
蜂王浆（RJ）的脂质组由中链（8 - 12个碳原子）游离脂肪酸组成。我们在此介绍一种液相色谱 - 高分辨率质谱（HRMS）方法，该方法可用于测定RJ脂肪酸，同时检测可疑脂肪酸。该方法允许直接定量RJ的七种游离脂肪酸，避免了任何衍生化步骤。它经过验证并应用于七个RJ样品，其中主要的RJ脂肪酸反式 - 10 - 羟基 - 2 - 癸烯酸（10 - HDA）在新鲜RJ中的含量为0.771 ± 0.08至0.928 ± 0.04 g/100 g。利用HRMS检测同时检测到另外四种可疑脂肪酸。
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