CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, Univ. Lille, F-59000 Lille, France.
Department of Veterinary Sciences, Food and Natural Resources, Faculty of Agrobiology, Czech University of Life Sciences in Prague, Kamycka 129, 16521 Prague-Suchdol, Czech Republic.
Cells. 2020 Jan 17;9(1):237. doi: 10.3390/cells9010237.
The role of hydrogen sulfide (HS) is addressed in oocytes. Three enzymes involved in HS metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous HS. On another hand, a dose-dependent inhibition was obtained using the HS donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to HS. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of HS signaling in the timing of amphibian oocytes meiosis resumption.
硫化氢（HS）在卵母细胞中的作用。三种参与 HS 代谢的酶，胱硫醚β-合酶、胱硫醚γ-裂合酶和 3-巯基丙酮酸硫转移酶，在前期 I 和中期 II 阻滞的卵母细胞中被检测到，并在被抑制时加速卵母细胞减数分裂恢复。此外，减数分裂恢复与内源性 HS 的显著减少有关。另一方面，使用 HS 供体 NaHS（1 和 5 mM）可获得剂量依赖性抑制。NaHS 抑制翻译。NaHS 不会诱导 M 期促进因子（MPF）的组成部分，即细胞周期蛋白 B 和 Cdk1 的解离，也不会直接影响 MPF 活性。然而，用中期 II MPF 包含的细胞质进行微注射诱导的 M 期进入减少，表明 MPF 自动放大环的上游组件对 HS 敏感。超氧化物歧化酶和过氧化氢酶阻碍了 NaHS 的作用，这种敏感性部分依赖于活性氧（ROS）的产生。与其他物种不同，没有促进细胞凋亡。这些结果表明 HS 信号在两栖类卵母细胞减数分裂恢复的时间控制中起作用。
