Krejcova Tereza, Smelcova Miroslava, Petr Jaroslav, Bodart Jean-Francois, Sedmikova Marketa, Nevoral Jan, Dvorakova Marketa, Vyskocilova Alena, Weingartova Ivona, Kucerova-Chrpova Veronika, Chmelikova Eva, Tumova Lenka, Jilek Frantisek
Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
Institute of Animal Science, Prague, Czech Republic.
PLoS One. 2015 Jan 23;10(1):e0116964. doi: 10.1371/journal.pone.0116964. eCollection 2015.
Porcine oocytes that have matured in in vitro conditions undergo the process of aging during prolonged cultivation, which is manifested by spontaneous parthenogenetic activation, lysis or fragmentation of aged oocytes. This study focused on the role of hydrogen sulfide (H2S) in the process of porcine oocyte aging. H2S is a gaseous signaling molecule and is produced endogenously by the enzymes cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MPST). We demonstrated that H2S-producing enzymes are active in porcine oocytes and that a statistically significant decline in endogenous H2S production occurs during the first day of aging. Inhibition of these enzymes accelerates signs of aging in oocytes and significantly increases the ratio of fragmented oocytes. The presence of exogenous H2S from a donor (Na2S.9H2O) significantly suppressed the manifestations of aging, reversed the effects of inhibitors and resulted in the complete suppression of oocyte fragmentation. Cultivation of aging oocytes in the presence of H2S donor positively affected their subsequent embryonic development following parthenogenetic activation. Although no unambiguous effects of exogenous H2S on MPF and MAPK activities were detected and the intracellular mechanism underlying H2S activity remains unclear, our study clearly demonstrates the role of H2S in the regulation of porcine oocyte aging.
在体外条件下成熟的猪卵母细胞在长时间培养过程中会经历老化过程，这表现为老化卵母细胞的自发孤雌激活、裂解或碎片化。本研究聚焦于硫化氢（H₂S）在猪卵母细胞老化过程中的作用。H₂S是一种气体信号分子，由胱硫醚-β-合酶（CBS）、胱硫醚-γ-裂解酶（CSE）和3-巯基丙酮酸硫转移酶（MPST）内源性产生。我们证明了产H₂S的酶在猪卵母细胞中具有活性，并且在老化的第一天内源性H₂S产生出现统计学上显著的下降。抑制这些酶会加速卵母细胞老化迹象，并显著增加碎片化卵母细胞的比例。来自供体（Na₂S·9H₂O）的外源性H₂S的存在显著抑制了老化表现，逆转了抑制剂的作用，并导致卵母细胞碎片化的完全抑制。在H₂S供体存在的情况下培养老化卵母细胞对其孤雌激活后的后续胚胎发育产生了积极影响。尽管未检测到外源性H₂S对MPF和MAPK活性有明确影响，且H₂S活性的细胞内机制仍不清楚，但我们的研究清楚地证明了H₂S在调节猪卵母细胞老化中的作用。
