Zhu Chen, Luo Xi, Espulgar Wilfred Villariza, Koyama Shohei, Kumanogoh Atsushi, Saito Masato, Takamatsu Hyota, Tamiya Eiichi
Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Micromachines (Basel). 2020 Jan 19;11(1):107. doi: 10.3390/mi11010107.
Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.
近几十年来，细胞因子分泌研究一直是科学家们研究的主要焦点，因为它对临床诊断有着突出贡献。局域表面等离子体共振（LSPR）技术是用于分析这些问题的传统方法之一，因为它可以对生物分子结合事件进行快速、无标记和实时监测。然而，过去许多基于LSPR的生物传感器通常用于监测细胞群体的平均性能，而不是单个细胞。同时，复杂的传感器结构会导致制造和经济预算问题。因此，在本文中，我们报告了一种简单的协同集成，即将微孔芯片的细胞捕获与金帽纳米柱结构的环烯烃聚合物（COP）膜相结合，用于单细胞水平的白细胞介素6（IL-6）检测。在这里，通过透射光谱中的峰值红移，可以直接观察和分析捕获细胞原位分泌的细胞因子。所制造的装置还显示出进行实时监测的潜力，这将极大地帮助我们识别被测单细胞的活力和生物学变化。
