致倦库蚊、白纹伊蚊和埃及伊蚊（双翅目：蚊科）成虫盘中期染色体核型分析

Analysis of the Metaphase Chromosome Karyotypes in Imaginal Discs of , , and (Diptera: Culicidae) Mosquitoes.

作者信息

Alekseeva Svetlana S, Andreeva Yulia V, Wasserlauf Irina E, Sibataev Anuarbek K, Stegniy Vladimir N

机构信息

Laboratory of Evolution Cytogenetics, Tomsk State University, Lenin st, 36, Tomsk 634050, Russia.

Laboratory of Evolutionary Genomics of Insects, the Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev ave., 10, Novosibirsk 630090, Russia.

出版信息

Insects. 2020 Jan 19;11(1):63. doi: 10.3390/insects11010063.

DOI:10.3390/insects11010063

PMID:31963900

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7022244/

Abstract

In this study, cytogenetic analysis of the metaphase chromosomes from imaginal discs of (Diptera: Culicidae) mosquitoes-, , , and -was performed. The patterns of C-banding and DAPI staining of the heteroсhromatin and the length of the chromosomes demonstrate species specificity. In particular, the chromosomes are the shortest compared with , , and , and they also carry additional C and DAPI bands in intercalary regions. The chromosomes are the longest, they have pericentromeric C bands, and they almost lack any DAPI bands near the centromere of chromosome 3 versus , which has the largest pericentromeric DAPI blocks in all three chromosome pairs. also possesses DAPI bands in the centromeric regions of all chromosomes, but their staining is weaker compared with those of Therefore, the analysis of karyotypes is a tool for species-level identification of these mosquitoes.

摘要

在本研究中，对伊蚊属（双翅目：蚊科）蚊虫——、、和——成虫盘中期染色体进行了细胞遗传学分析。异染色质的C带和DAPI染色模式以及染色体长度显示出物种特异性。特别是，染色体与、和相比最短，并且在居间区域还带有额外的C带和DAPI带。染色体最长，它们有近着丝粒C带，并且与相比，其在3号染色体着丝粒附近几乎没有任何DAPI带，而在所有三对染色体中，具有最大的近着丝粒DAPI块。在所有染色体的着丝粒区域也具有DAPI带，但其染色与的相比更弱。因此，核型分析是这些蚊虫物种水平鉴定的一种工具。