Li Junying, Liang Zongan, Zheng Jun, Zhang Xiaohong, Fu Jing
Department of Respiratory Medicine, West China School of Medicine, Sichuan University Chengdu 610041, P. R. China.
Department of Emergency Medicine, Sichuan Provincial People's Hospital Chengdu 610032, P. R. China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):10910-10917. eCollection 2017.
Long non-coding RNAs (lncRNAs) are critical gene regulators with important roles in a wide variety of biological processes, including tumorigenesis. ZEB1-AS1 was significantly increased in NSCLC tissues and cell lines, and its expression levels were highly associated with aggressive tumor progression and poor prognosis. Down-regulation of ZEB1-AS1 suppresses NSCLC cell proliferation and invasion in vitro, as well as inhibits xenograft tumor growth in vivo. Mechanistically, we found that ZEB1-AS1 down-regulation effectively suppressed EMT process through reducing ZEB1 expression, and the ectopic expression of ZEB1 restored the migratory and invasive abilities of NSCLC cells inhibited by ZEB1-AS1 down-regulation. In conclusion, these findings revealed that ZEB1-AS1 plays regulatory roles in NSCLC and it might become a novel molecular indicator of prognosis and therapeutic target in NSCLC.
长链非编码RNA（lncRNAs）是关键的基因调节因子，在包括肿瘤发生在内的多种生物学过程中发挥重要作用。ZEB1-AS1在非小细胞肺癌（NSCLC）组织和细胞系中显著上调，其表达水平与肿瘤的侵袭性进展和不良预后高度相关。下调ZEB1-AS1可抑制NSCLC细胞的体外增殖和侵袭，并抑制体内异种移植肿瘤的生长。机制上，我们发现下调ZEB1-AS1可通过降低ZEB1的表达有效抑制上皮-间质转化（EMT）过程，而ZEB1的异位表达可恢复下调ZEB1-AS1所抑制的NSCLC细胞的迁移和侵袭能力。总之，这些发现表明ZEB1-AS1在NSCLC中发挥调节作用，它可能成为NSCLC预后的新型分子指标和治疗靶点。
