Gao Shan, Guo Jiayi, Li Feng, Zhang Kun, Zhang Yuke, Zhang Yunfei, Guo Yanxing
Hunan University of Chinese Medicine Changsha, Hunan, China.
Luoyang Orthopedic Hospital of Henan Province Luoyang, Henan, China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):10918-10925. eCollection 2017.
The 5-year survival rate of patients with metastatic osteosarcoma remains poor. Therefore, the molecular mechanisms underlying metastasis of osteosarcoma need to be investigated. Long non-coding RNA promotes tumor metastasis in liver and lung cancers; however, its role in osteosarcoma remains unclear. In this study, we found that expression was significantly higher in osteosarcoma tissues than that in adjacent normal osteosarcoma tissues and upregulated expression was significantly correlated with tumor metastasis, higher TNM grade and lower survival rate. Additionally, we observed that silencing significantly inhibited the migration and invasion of osteosarcoma cells, but showed no effects on the proliferation and apoptosis of these cells. silencing markedly increased the expression of E-cadherin and decreased the expressions of N-cadherin, vimentin, matrix metalloproteinase-2 (MMP-2), and MMP-9, which exerted a potentiating effect on EMT. The result was suggested that silencing inhibited tumor metastasis in osteosarcoma by possibly inhibiting EMT process. In conclusion, these observations indicated the potential of as a biomarker of poor prognosis and promising target for treating osteosarcoma.
转移性骨肉瘤患者的5年生存率仍然很低。因此，需要研究骨肉瘤转移的分子机制。长链非编码RNA促进肝癌和肺癌的肿瘤转移；然而，其在骨肉瘤中的作用仍不清楚。在本研究中，我们发现其在骨肉瘤组织中的表达明显高于相邻正常骨肉瘤组织，且上调的表达与肿瘤转移、更高的TNM分级和更低的生存率显著相关。此外，我们观察到其沉默显著抑制了骨肉瘤细胞的迁移和侵袭，但对这些细胞的增殖和凋亡没有影响。其沉默显著增加了E-钙黏蛋白的表达，降低了N-钙黏蛋白、波形蛋白、基质金属蛋白酶-2（MMP-2）和MMP-9的表达，对上皮-间质转化（EMT）产生了增强作用。结果表明，其沉默可能通过抑制EMT过程来抑制骨肉瘤的肿瘤转移。总之，这些观察结果表明其作为预后不良生物标志物的潜力以及作为治疗骨肉瘤的有前景靶点的可能性。
