Qin Zhiqiang, Li Xiao, Yang Jie, Cao Pu, Qin Chao, Xue Jianxin, Jia Ruipeng
Department of Urology, The Second Affiliated Hospital of Southeast University Nanjing 210003, China.
Department of Urology, The First Affiliated Hospital of Nanjing Medical University Nanjing 210029, China.
Int J Clin Exp Pathol. 2017 Dec 1;10(12):11896-11908. eCollection 2017.
The aim of this study was to determine whether vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) promoted the mobilization and recruitment of endothelial progenitor cells (EPCs) to protect kidneys from ischemia and reperfusion injury (IRI) in male rats. At 24 h and 72 h after reperfusion, serum samples were respectively collected for renal function. Besides, kidney tissues were harvested to observe renal morphology changes. Subsequently, VEGF, Ang-1 and angiopoietin-2 (Ang-2) expression levels in different groups were measured at the indicated time points after reperfusion. Compared with IRI-operated group, rats that were intervened with EPCs significantly reduced in the levels of blood urea nitrogen, serum creatinine at 24 hours and 72 hours, particularly in injecting EPCs suspension liquid transfected by VEGF-adenovirus and Ang-1-adenovirus. At 72 hours after reperfusion, renal function and morphology were exhibited significant improvements in two EPCs-transfected VEGF-adenovirus and Ang-1-adenovirus groups. In addition, expression levels of VEGF, Ang-1 and Ang-2 in the kidneys of EPCs-treated rats which were transfected by VEGF-adenovirus and Ang-1-adenovirus were markedly increased compared to rats subjected to IRI. The present work suggested that VEGF and Ang-1 might play important roles in the protective effect of homing of EPCs on renal acute IRI.
本研究旨在确定血管内皮生长因子（VEGF）和血管生成素-1（Ang-1）是否能促进内皮祖细胞（EPCs）的动员和募集，以保护雄性大鼠的肾脏免受缺血再灌注损伤（IRI）。再灌注后24小时和72小时，分别采集血清样本检测肾功能。此外，取肾组织观察肾脏形态变化。随后，在再灌注后的指定时间点测量不同组中VEGF、Ang-1和血管生成素-2（Ang-2）的表达水平。与IRI手术组相比，接受EPCs干预的大鼠在24小时和72小时时血尿素氮、血清肌酐水平显著降低，特别是注射经VEGF腺病毒和Ang-1腺病毒转染的EPCs悬浮液的大鼠。再灌注72小时后，两个EPCs转染VEGF腺病毒和Ang-1腺病毒组的肾功能和形态均有显著改善。此外，与IRI大鼠相比，经VEGF腺病毒和Ang-1腺病毒转染的EPCs处理大鼠肾脏中VEGF、Ang-1和Ang-2的表达水平明显升高。目前的研究表明，VEGF和Ang-1可能在EPCs归巢对肾脏急性IRI的保护作用中发挥重要作用。
