Competence Center for Biocatalysis, Institute for Chemistry and Biotechnology, Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820, Wädenswil, Switzerland.
Chemistry. 2020 Jun 10;26(33):7336-7345. doi: 10.1002/chem.201905752. Epub 2020 Apr 15.
Freestanding Fe/α-ketoglutarate-dependent halogenases are oxidoreductases that catalyze the installation of halogen atoms into unactivated sp -hybridized carbon centers with high stereo- and regioselectivity. Since their discovery in 2014, a small number of indole alkaloid and amino acid halogenases have been identified and characterized. First enzyme engineering examples suggest that the accessible substrate range of these enzymes may be expanded through the use of rational enzyme design and directed evolution. Structural investigations of non-heme iron halogenases acting on freestanding as well as tethered substrates are beginning to inform about the principles of the underlying halogenation mechanism.
游离态的 Fe/α-酮戊二酸依赖型卤代酶是氧化还原酶，能够以高立体和区域选择性将卤原子催化安装到未活化的 sp 杂化碳中心。自 2014 年发现以来，已经鉴定和表征了少数吲哚生物碱和氨基酸卤代酶。初步的酶工程实例表明，通过合理的酶设计和定向进化，可以扩展这些酶的可及底物范围。对作用于游离和连接底物的非血红素铁卤代酶的结构研究开始阐明潜在卤化机制的原理。
